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Cotranscription of the Genes Encoding Two P700 Chlorophyll a Apoproteins with the Gene for Ribosomal Protein CS14: Determination of the Transcriptional Initiation Site by in Vitro Capping

Overview
Journal Curr Genet
Specialty Genetics
Date 1988 Oct 1
PMID 2850871
Citations 27
Authors
B Y Meng
M Tanaka
T Wakasugi
M Ohme
K Shinozaki
M Sugiura
Affiliations
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Abstract

Transcription of the psaA operon in tobacco chloroplasts has been studied. This operon contains in linear sequence the genes encoding the P700 chlorophyll a A1 and A2 apoproteins (psaA and psaB) and the gene encoding the ribosomal CS14 protein (rps14). Northern blot hybridization revealed that a 5.2 kb transcript hybridizes to psaA, psaB, and rps14, but not to the fMet-tRNA (trnfM) gene which follows. Primer extension and in vitro capping assays indicated that the transcriptional initiation site is 194 bp upstream of psaA. The 3' end of the transcript was determined by S1 mapping to be 105 bp downstream of rps14. The transcript is calculated to be 5,207 nucleotides long.

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References
1.
Westhoff P, Alt J, Nelson N, Bottomley W, Bunemann H, Herrmann R . Genes and transcripts for the P700 chlorophylla apoprotein and subunit 2 of the photosystem I reaction center complex from spinach thylakoid membranes. Plant Mol Biol. 2013; 2(2):95-107. DOI: 10.1007/BF01595171. View
2.
Fish L, Kuck U, Bogorad L . Two partially homologous adjacent light-inducible maize chloroplast genes encoding polypeptides of the P700 chlorophyll a-protein complex of photosystem I. J Biol Chem. 1985; 260(3):1413-21. View
3.
Berends T, Gamble P, Mullet J . Characterization of the barley chloroplast transcription units containing psaA-psaB and psbD-psbC. Nucleic Acids Res. 1987; 15(13):5217-40. PMC: 305957. DOI: 10.1093/nar/15.13.5217. View
4.
Lehmbeck J, Rasmussen O, Bookjans G, Jepsen B, Stummann B, Henningsen K . Sequence of two genes in pea chloroplast DNA coding for 84 and 82 kD polypeptides of the photosystem I complex. Plant Mol Biol. 2013; 7(1):3-10. DOI: 10.1007/BF00020126. View
5.
Shinozaki K, Sugiura M . The nucleotide sequence of the tobacco chloroplast gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Gene. 1982; 20(1):91-102. DOI: 10.1016/0378-1119(82)90090-7. View