Proteomics Show Antigen Presentation Processes in Human Immune Cells After AS03-H5N1 Vaccination

Overview

Journal Proteomics

Date 2017 May 17

PMID 28508465

Citations 5

Authors

Allison C Galassie

Johannes B Goll

Parimal Samir

Travis L Jensen

Kristen L Hoek

Leigh M Howard

Tara M Allos

Xinnan Niu

Laura E Gordy

C Buddy Creech

Heather Hill

Sebastian Joyce

Kathryn M Edwards

Andrew J Link

Affiliations

Soon will be listed here.

Abstract

Adjuvants enhance immunity elicited by vaccines through mechanisms that are poorly understood. Using a systems biology approach, we investigated temporal protein expression changes in five primary human immune cell populations: neutrophils, monocytes, natural killer cells, T cells, and B cells after administration of either an Adjuvant System 03 adjuvanted or unadjuvanted split-virus H5N1 influenza vaccine. Monocytes demonstrated the strongest differential signal between vaccine groups. On day 3 post-vaccination, several antigen presentation-related pathways, including MHC class I-mediated antigen processing and presentation, were enriched in monocytes and neutrophils and expression of HLA class I proteins was increased in the Adjuvant System 03 group. We identified several protein families whose proteomic responses predicted seroprotective antibody responses (>1:40 hemagglutination inhibition titer), including inflammation and oxidative stress proteins at day 1 as well as immunoproteasome subunit (PSME1 and PSME2) and HLA class I proteins at day 3 in monocytes. While comparison between temporal proteomic and transcriptomic results showed little overlap overall, enrichment of the MHC class I antigen processing and presentation pathway in monocytes and neutrophils was confirmed by both approaches.

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