R-Ras Deficiency Does Not Affect Papain-induced IgE Production in Mice

Overview

Journal Immun Inflamm Dis

Specialties Allergy & Immunology
Infectious Diseases

Date 2017 May 13

PMID 28497586

Citations 2

Authors

Laura Kummola

Zsuzsanna Ortutay

Maria Vahatupa

Stuart Prince

Hannele Uusitalo-Jarvinen

Tero A H Jarvinen

Ilkka S Junttila

Affiliations

Soon will be listed here.

Abstract

Introduction: R-Ras GTPase has recently been implicated in the regulation of immune functions, particularly in dendritic cell (DC) maturation, immune synapse formation, and subsequent T cell responses.

Methods: Here, we investigated the role of R-Ras in allergen-induced immune response (type 2 immune response) in Rras deficient (R-Ras KO) and wild type (WT) mice.

Results: Initially, we found that the number of conventional DC's in the lymph nodes (LNs) was reduced in R-Ras KO mice. The expression of co-stimulatory CD80 and CD86 molecules on these cells was also reduced on DC's from the R-Ras KO mice. However, there was no difference in papain-induced immune response between the R-Ras WT and KO as measured by serum IgE levels after the immunization. Interestingly, neither the DC number nor co-stimulatory molecule expression was different between WT and R-Ras KO animals after the immunization.

Conclusions: Taken together, despite having reduced number of conventional DC's in the R-Ras KO mice and low expression of CD80 on DC's, the R-Ras KO mice are capable of mounting papain-induced IgE responses comparable to that of the WT mice. To our knowledge, this is the first report addressing potential differences in in vivo allergen responses regulated by the R-Ras GTPase.

Citing Articles

Proteomics Analysis of R-Ras Deficiency in Oxygen Induced Retinopathy.

Vahatupa M, Nattinen J, Aapola U, Uusitalo-Jarvinen H, Uusitalo H, Jarvinen T Int J Mol Sci. 2023; 24(9).

PMID: 37175621 PMC: 10178533. DOI: 10.3390/ijms24097914.

Selective Targeting and Tissue Penetration to the Retina by a Systemically Administered Vascular Homing Peptide in Oxygen Induced Retinopathy (OIR).

Vahatupa M, Salonen N, Uusitalo-Jarvinen H, Jarvinen T Pharmaceutics. 2021; 13(11).

PMID: 34834347 PMC: 8618640. DOI: 10.3390/pharmaceutics13111932.

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