Effects of Quercetin and Mannitol on Erythropoietin Levels in Rats Following Acute Severe Traumatic Brain Injury

Overview

Journal J Korean Neurosurg Soc

Date 2017 May 12

PMID 28490163

Citations 8

Authors

Orhan Kalemci

Hasan Emre Aydin

Ceren Kizmazoglu

Ismail Kaya

Hulya Yilmaz

Nuri M Arda

Affiliations

Soon will be listed here.

Abstract

Objective: The aim of this study to investigate the normal values of erythropoietin (EPO) and neuroprotective effects of quercetin and mannitol on EPO and hematocrit levels after acute severe traumatic brain injury (TBI) in rat model.

Methods: A weight-drop impact acceleration model of TBI was used on 40 male Wistar rats. The animals were divided into sham (group I), TBI (group II), TBI+quercetin (50 mg/kg intravenously) (group III), and TBI+mannitol (1 mg/kg intravenously) (group IV) groups. The malondialdehyde, glutathione peroxidase, catalase, EPO, and hematocrit levels were measured 1 and 4 hour after injury. Two-way repeated measures analysis of variance and Tukey's test were used for statistical analysis.

Results: The malondialdehyde levels decreased significantly after administration of quercetin and mannitol compared with those in group II. Catalase and glutathione peroxidase levels increased significantly in groups III and IV. Serum EPO levels decreased significantly after mannitol but not after quercetin administration. Serum hematocrit levels did not change significantly after quercetin and mannitol administration 1 hour after trauma. However, mannitol administration decreased serum hematocrit levels significantly after 4 hour.

Conclusion: This study suggests that quercetin may be a good alternative treatment for TBI, as it did not decrease the EPO levels.

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References

Bischoff S . Quercetin: potentials in the prevention and therapy of disease. Curr Opin Clin Nutr Metab Care. 2008; 11(6):733-40. DOI: 10.1097/MCO.0b013e32831394b8. View

Peng W, Xing Z, Yang J, Wang Y, Wang W, Huang W . The efficacy of erythropoietin in treating experimental traumatic brain injury: a systematic review of controlled trials in animal models. J Neurosurg. 2014; 121(3):653-64. DOI: 10.3171/2014.6.JNS132577. View

Zhang Y, Xiong Y, Mahmood A, Meng Y, Qu C, Schallert T . Therapeutic effects of erythropoietin on histological and functional outcomes following traumatic brain injury in rats are independent of hematocrit. Brain Res. 2009; 1294:153-64. PMC: 2746872. DOI: 10.1016/j.brainres.2009.07.077. View

Bouzat P, Millet A, Boue Y, Pernet-Gallay K, Trouve-Buisson T, Gaide-Chevronnay L . Changes in brain tissue oxygenation after treatment of diffuse traumatic brain injury by erythropoietin. Crit Care Med. 2013; 41(5):1316-24. DOI: 10.1097/CCM.0b013e31827ca64e. View

Myhrstad M, Carlsen H, Nordstrom O, Blomhoff R, Moskaug J . Flavonoids increase the intracellular glutathione level by transactivation of the gamma-glutamylcysteine synthetase catalytical subunit promoter. Free Radic Biol Med. 2002; 32(5):386-93. DOI: 10.1016/s0891-5849(01)00812-7. View

MIDDLETON Jr E, Kandaswami C, Theoharides T . The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol Rev. 2000; 52(4):673-751. View

Faria A, Pestana D, Teixeira D, Azevedo J, de Freitas V, Mateus N . Flavonoid transport across RBE4 cells: A blood-brain barrier model. Cell Mol Biol Lett. 2010; 15(2):234-41. PMC: 6275689. DOI: 10.2478/s11658-010-0006-4. View

Tango H, Schmidt A, Mizumoto N, Lacava M, Cruz Jr R, Cruz Jr J . Low hematocrit levels increase intracranial pressure in an animal model of cryogenic brain injury. J Trauma. 2009; 66(3):720-6. DOI: 10.1097/TA.0b013e3181719b35. View

Lee J, Lee C, Kim H, Lee C, Kim H, Kim J . A Role of Serum-Based Neuronal and Glial Markers as Potential Predictors for Distinguishing Severity and Related Outcomes in Traumatic Brain Injury. J Korean Neurosurg Soc. 2015; 58(2):93-100. PMC: 4564754. DOI: 10.3340/jkns.2015.58.2.93. View

10.

Yilmaz N, Dulger H, Kiymaz N, Yilmaz C, Gudu B, Demir I . Activity of mannitol and hypertonic saline therapy on the oxidant and antioxidant system during the acute term after traumatic brain injury in the rats. Brain Res. 2007; 1164:132-5. DOI: 10.1016/j.brainres.2007.06.017. View

11.

Youdim K, Dobbie M, Gunter Kuhnle , Proteggente A, Abbott N, Rice-Evans C . Interaction between flavonoids and the blood-brain barrier: in vitro studies. J Neurochem. 2003; 85(1):180-92. DOI: 10.1046/j.1471-4159.2003.01652.x. View

12.

Schober M, Requena D, Block B, Davis L, Rodesch C, Casper T . Erythropoietin improved cognitive function and decreased hippocampal caspase activity in rat pups after traumatic brain injury. J Neurotrauma. 2013; 31(4):358-69. PMC: 3922399. DOI: 10.1089/neu.2013.2922. View

13.

Fisher J . Erythropoietin: physiology and pharmacology update. Exp Biol Med (Maywood). 2003; 228(1):1-14. DOI: 10.1177/153537020322800101. View

14.

Haleagrahara N, Radhakrishnan A, Lee N, Kumar P . Flavonoid quercetin protects against swimming stress-induced changes in oxidative biomarkers in the hypothalamus of rats. Eur J Pharmacol. 2009; 621(1-3):46-52. DOI: 10.1016/j.ejphar.2009.08.030. View

15.

Rossi R, Dalle-Donne I, Milzani A, Giustarini D . Oxidized forms of glutathione in peripheral blood as biomarkers of oxidative stress. Clin Chem. 2006; 52(7):1406-14. DOI: 10.1373/clinchem.2006.067793. View

16.

Hartley C, Varma M, Fischer J, Riccardi R, Strauss J, Shah S . Neuroprotective effects of erythropoietin on acute metabolic and pathological changes in experimentally induced neurotrauma. J Neurosurg. 2008; 109(4):708-14. DOI: 10.3171/JNS/2008/109/10/0708. View

17.

Harnly J, Doherty R, Beecher G, Holden J, Haytowitz D, Bhagwat S . Flavonoid content of U.S. fruits, vegetables, and nuts. J Agric Food Chem. 2006; 54(26):9966-77. DOI: 10.1021/jf061478a. View

18.

Rangel-Ordonez L, Noldner M, Schubert-Zsilavecz M, Wurglics M . Plasma levels and distribution of flavonoids in rat brain after single and repeated doses of standardized Ginkgo biloba extract EGb 761®. Planta Med. 2010; 76(15):1683-90. DOI: 10.1055/s-0030-1249962. View

19.

Velly L, Pellegrini L, Guillet B, Bruder N, Pisano P . Erythropoietin 2nd cerebral protection after acute injuries: a double-edged sword?. Pharmacol Ther. 2010; 128(3):445-59. DOI: 10.1016/j.pharmthera.2010.08.002. View

20.

Ferrali M, Signorini C, Ciccoli L, Bambagioni S, Rossi V, Pompella A . Protection of erythrocytes against oxidative damage and autologous immunoglobulin G (IgG) binding by iron chelator fluor-benzoil-pyridoxal hydrazone. Biochem Pharmacol. 2001; 59(11):1365-73. DOI: 10.1016/s0006-2952(00)00273-2. View