Bam-dependent Deubiquitinase Complex Can Disrupt Germ-line Stem Cell Maintenance by Targeting Cyclin A

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2017 May 10

PMID 28484036

Citations 27

Authors

Shanming Ji

Chaoyi Li

Lin Hu

Kehui Liu

Jie Mei

Yuewan Luo

Yi Tao

Zongping Xia

Qinmiao Sun

Dahua Chen

Affiliations

Soon will be listed here.

Abstract

germ-line stem cells (GSCs) provide an excellent model to study the regulatory mechanisms of stem cells in vivo. () has been demonstrated to be necessary and sufficient to promote GSC and cystoblast differentiation. Despite extensive investigation of its regulation and genetic functions, the biochemical nature of the Bam protein has been unknown. Here, we report that Bam is an ubiquitin-associated protein and controls the turnover of cyclin A (CycA). Mechanistically, we found that Bam associated with Otu to form a deubiquitinase complex that stabilized CycA by deubiquitination, thus providing a mechanism to explain how ectopic expression of Bam in GSCs promotes differentiation. Collectively, our findings not only identify a biochemical function of Bam, which contributes to GSC fate determination, but also emphasizes the critical role of proper expression of cyclin proteins mediated by both ubiquitination and deubiquitination pathways in balancing stem cell self-renewal and differentiation.

Citing Articles

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