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HUCMNCs Protect Vascular Endothelium and Prevent ISR After Endovascular Interventional Therapy for Vascular Diseases in T2DM Rabbits

Overview
Publisher Springer
Specialty Biochemistry
Date 2017 May 6
PMID 28474283
Citations 2
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Abstract

The therapeutic effect of transplantation of human umbilical cord blood cell-derived mononuclear cells (HUCMNCs) on treating in-stent restenosis (ISR) after endovascular interventional therapy (EIT) was evaluated in preclinical rabbit model of type 2 diabetes mellitus (T2DM)-related peripheral artery disease (PAD). HUCMNCs were transplanted to T2DM rabbits subjected to femoral artery occlusion surgery and received EIT. Serum concentration of soluble vascular endothelial cadherin (VE-cad) and plasma concentration of lipoprotein-associated phospholipase A2 (Lp-PLA2) were determined with enzyme-linked immunosorbent assay before and after the transplantation. The injury and the recovery of right femoral artery at stenting site were evaluated with Hematoxylin and Eosin (HE) staining. HUCMNCs purified from umbilical cord blood were 100% CD45 and 96.5% CD34 with round or oval morphology and adherent growth pattern. The soluble VE-cad and Lp-PLA2 were significantly attenuated after HUCMNC transplantation. The intimal area and the ratio between intimal area and medium film area in the dilated occlusion site were also dramatically decreased 4 weeks after receiving HUCMNCs. HUCMNC transplantation is effective in protecting vascular endothelial function and preventing ISR after EIT in T2DM rabbits suffering from PAD.

Citing Articles

Effect of umbilical cord blood stem cell transplantation on restenosis after endovascular interventional therapy for diabetic hindlimb vascular disease.

Ding H, Xing N, Ma H, Hou L, Zhou C, Du Y PLoS One. 2021; 16(8):e0255162.

PMID: 34379650 PMC: 8357084. DOI: 10.1371/journal.pone.0255162.


The effect of endothelial progenitor cell transplantation on neointimal hyperplasia and reendothelialisation after balloon catheter injury in rat carotid arteries.

Wang W, Zhang Y, Hui H, Tong W, Wei Z, Li Z Stem Cell Res Ther. 2021; 12(1):99.

PMID: 33536065 PMC: 7860581. DOI: 10.1186/s13287-021-02135-w.

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