Anticonvulsant and Neuroprotective Activities of Extract in Pentylenetetrazole-Kindled Mice

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2017 May 4

PMID 28465705

Citations 5

Authors

Hibah M Aldawsari

Basma G Eid

Thikrayat Neamatallah

Sawsan A Zaitone

Jihan M Badr

Affiliations

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Abstract

Anticonvulsant and neuroprotective activity of extract were tested in pentylenetetrazole-kindled mice. All the chemical constituents of the plant extract were identified. Additionally, the extract was standardized and proved to contain total phenolic contents equal to 379.92 ± 1.32 mg gallic acid equivalents/g dry plant extract. Induction of kindling was achieved by repeated intraperitoneal administration of pentylenetetrazole (35 mg/kg) twice weekly. Male albino mice were given . extract (200, 400, or 800 mg/kg). The two higher doses (400 or 800 mg/kg) of the extract significantly caused notable reduction in seizure activity and hippocampal malondialdehyde level compared to pentylenetetrazole control group. The highest dose enhanced cortical GSH level and showed intact DNA in the laddering assay. Upon studying the neuroprotective effect, mice treated with the higher dose of the extract demonstrated an improvement in the percent of surviving neurons in the cortex and hippocampus. We concluded that extract ameliorated seizure activity and protected cortical and hippocampal neurons against pentylenetetrazole-induced kindling in mice.

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References

Taiwe G, Moto F, Ayissi E, Ngoupaye G, Njapdounke J, Nkantchoua G . Effects of a lyophilized aqueous extract of Feretia apodanthera Del. (Rubiaceae) on pentylenetetrazole-induced kindling, oxidative stress, and cognitive impairment in mice. Epilepsy Behav. 2015; 43:100-8. DOI: 10.1016/j.yebeh.2014.11.022. View

Duncan J . Seizure-induced neuronal injury: human data. Neurology. 2002; 59(9 Suppl 5):S15-20. DOI: 10.1212/wnl.59.9_suppl_5.s15. View

Coyle J, Puttfarcken P . Oxidative stress, glutamate, and neurodegenerative disorders. Science. 1993; 262(5134):689-95. DOI: 10.1126/science.7901908. View

Ghabaee M, Jabedari B, Al-E-Eshagh N, Ghaffarpour M, Asadi F . Serum and cerebrospinal fluid antioxidant activity and lipid peroxidation in Guillain-Barre syndrome and multiple sclerosis patients. Int J Neurosci. 2010; 120(4):301-4. DOI: 10.3109/00207451003695690. View

Swarnkar S, Singh S, Sharma S, Mathur R, Patro I, Nath C . Rotenone induced neurotoxicity in rat brain areas: a histopathological study. Neurosci Lett. 2011; 501(3):123-7. DOI: 10.1016/j.neulet.2011.03.036. View

Alhaj M, Zaitone S, Moustafa Y . Fluvoxamine alleviates seizure activity and downregulates hippocampal GAP-43 expression in pentylenetetrazole-kindled mice: role of 5-HT3 receptors. Behav Pharmacol. 2015; 26(4):369-82. DOI: 10.1097/FBP.0000000000000127. View

Zhang R, Lu H, Tian S, Yin J, Chen Q, Ma L . Protective effects of pre-germinated brown rice diet on low levels of Pb-induced learning and memory deficits in developing rat. Chem Biol Interact. 2010; 184(3):484-91. DOI: 10.1016/j.cbi.2010.01.043. View

Lei X, Chao H, Zhang Z, Lv J, Li S, Wei H . Neuroprotective effects of quercetin in a mouse model of brain ischemic/reperfusion injury via anti-apoptotic mechanisms based on the Akt pathway. Mol Med Rep. 2015; 12(3):3688-3696. DOI: 10.3892/mmr.2015.3857. View

Lin M, Beal M . Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. Nature. 2006; 443(7113):787-95. DOI: 10.1038/nature05292. View

10.

Ramzan I . High-performance liquid chromatographic determination of chemical convulsant pentylenetetrazol in rat serum, cerebrospinal fluid and brain. J Chromatogr. 1988; 432:370-4. DOI: 10.1016/s0378-4347(00)80668-4. View

11.

Nunomura A, Perry G, Aliev G, Hirai K, Takeda A, Balraj E . Oxidative damage is the earliest event in Alzheimer disease. J Neuropathol Exp Neurol. 2001; 60(8):759-67. DOI: 10.1093/jnen/60.8.759. View

12.

Aldawsari H, Hanafy A, Labib G, Badr J . Antihyperglycemic activities of extracts of the mistletoes Plicosepalus acaciae and P. curviflorus in comparison to their solid lipid nanoparticle suspension formulations. Z Naturforsch C J Biosci. 2015; 69(9-10):391-8. DOI: 10.5560/znc.2014-0047. View

13.

Racine R . Modification of seizure activity by electrical stimulation. II. Motor seizure. Electroencephalogr Clin Neurophysiol. 1972; 32(3):281-94. DOI: 10.1016/0013-4694(72)90177-0. View

14.

Teema A, Zaitone S, Moustafa Y . Ibuprofen or piroxicam protects nigral neurons and delays the development of l-dopa induced dyskinesia in rats with experimental Parkinsonism: Influence on angiogenesis. Neuropharmacology. 2016; 107:432-450. DOI: 10.1016/j.neuropharm.2016.03.034. View

15.

Sun J, Li Y, Ding Y, Wang J, Geng J, Yang H . Neuroprotective effects of gallic acid against hypoxia/reoxygenation-induced mitochondrial dysfunctions in vitro and cerebral ischemia/reperfusion injury in vivo. Brain Res. 2014; 1589:126-39. DOI: 10.1016/j.brainres.2014.09.039. View

16.

Liu T, Jin H, Sun Q, Xu J, Hu H . Neuroprotective effects of emodin in rat cortical neurons against beta-amyloid-induced neurotoxicity. Brain Res. 2010; 1347:149-60. DOI: 10.1016/j.brainres.2010.05.079. View

17.

Gupta Y, Veerendra Kumar M, Srivastava A . Effect of Centella asiatica on pentylenetetrazole-induced kindling, cognition and oxidative stress in rats. Pharmacol Biochem Behav. 2003; 74(3):579-85. DOI: 10.1016/s0091-3057(02)01044-4. View

18.

Sutula T, Hagen J, Pitkanen A . Do epileptic seizures damage the brain?. Curr Opin Neurol. 2003; 16(2):189-95. DOI: 10.1097/01.wco.0000063770.15877.bc. View

19.

Castellani R, Harris P, Sayre L, Fujii J, Taniguchi N, Vitek M . Active glycation in neurofibrillary pathology of Alzheimer disease: N(epsilon)-(carboxymethyl) lysine and hexitol-lysine. Free Radic Biol Med. 2001; 31(2):175-80. DOI: 10.1016/s0891-5849(01)00570-6. View

20.

Henchcliffe C, Beal M . Mitochondrial biology and oxidative stress in Parkinson disease pathogenesis. Nat Clin Pract Neurol. 2008; 4(11):600-9. DOI: 10.1038/ncpneuro0924. View