Metabolic Engineering of Carotenoids in Transgenic Sweetpotato

Overview

Journal Breed Sci

Date 2017 May 4

PMID 28465665

Citations 12

Authors

Le Kang

Sung-Chul Park

Chang Yoon Ji

Ho Soo Kim

Haeng-Soon Lee

Sang-Soo Kwak

Affiliations

Soon will be listed here.

Abstract

Sweetpotato [ (L.) Lam], which contains high levels of antioxidants such as ascorbate and carotenoids in its storage root, is one of the healthiest foods, as well as one of the best starch crops for growth on marginal lands. In plants, carotenoid pigments are involved in light harvesting for photosynthesis and are also essential for photo-protection against excess light. As dietary antioxidants in humans, these compounds benefit health by alleviating aging-related diseases. The storage root of sweetpotato is a good source of both carotenoids and carbohydrates for human consumption. Therefore, metabolic engineering of sweetpotato to increase the content of useful carotenoids represents an important agricultural goal. This effort has been facilitated by cloning of most of the carotenoid biosynthetic genes, as well as the gene involved in carotenoid accumulation. In this review, we describe our current understanding of the regulation of biosynthesis, accumulation and catabolism of carotenoids in sweetpotato. A deeper understanding of these topics should contribute to development of new sweetpotato cultivars with higher levels of nutritional carotenoids and abiotic stress tolerance.

Citing Articles

A comprehensive overview of omics-based approaches to enhance biotic and abiotic stress tolerance in sweet potato.

Ahmed S, Khan M, Xue S, Islam F, Ikram A, Abdullah M Hortic Res. 2024; 11(3):uhae014.

PMID: 38464477 PMC: 10923648. DOI: 10.1093/hr/uhae014.

The transcription factor IbNAC29 positively regulates the carotenoid accumulation in sweet potato.

Xing S, Li R, Zhao H, Zhai H, He S, Zhang H Hortic Res. 2023; 10(3):uhad010.

PMID: 36960431 PMC: 10028406. DOI: 10.1093/hr/uhad010.

Chemistry, Occurrence, Properties, Applications, and Encapsulation of Carotenoids-A Review.

Gonzalez-Pena M, Ortega-Regules A, de Parrodi C, Lozada-Ramirez J Plants (Basel). 2023; 12(2).

PMID: 36679026 PMC: 9865331. DOI: 10.3390/plants12020313.

Integrated Metabolomic and Transcriptomic Analyses Reveal the Basis for Carotenoid Biosynthesis in Sweet Potato ( (L.) Lam.) Storage Roots.

Ren Q, Zhen X, Gao H, Liang Y, Li H, Zhao J Metabolites. 2022; 12(11).

PMID: 36355093 PMC: 9699360. DOI: 10.3390/metabo12111010.

Integrated analysis of carotenoid metabolites and transcriptome identifies key genes controlling carotenoid compositions and content in sweetpotato tuberous roots ( L.).

Jia R, Zhang R, Gangurde S, Tang C, Jiang B, Li G Front Plant Sci. 2022; 13:993682.

PMID: 36340393 PMC: 9632283. DOI: 10.3389/fpls.2022.993682.

References

Park S, Kim S, Park S, Lee H, Lee J, Park W . Enhanced accumulation of carotenoids in sweetpotato plants overexpressing IbOr-Ins gene in purple-fleshed sweetpotato cultivar. Plant Physiol Biochem. 2014; 86:82-90. DOI: 10.1016/j.plaphy.2014.11.017. View

Campbell R, Ducreux L, Morris W, Morris J, Suttle J, Ramsay G . The metabolic and developmental roles of carotenoid cleavage dioxygenase4 from potato. Plant Physiol. 2010; 154(2):656-64. PMC: 2949026. DOI: 10.1104/pp.110.158733. View

Cunningham F, Gantt E . GENES AND ENZYMES OF CAROTENOID BIOSYNTHESIS IN PLANTS. Annu Rev Plant Physiol Plant Mol Biol. 2004; 49:557-583. DOI: 10.1146/annurev.arplant.49.1.557. View

Tata S, Jung J, Kim Y, Choi J, Jung J, Lee I . Heterologous expression of chloroplast-localized geranylgeranyl pyrophosphate synthase confers fast plant growth, early flowering and increased seed yield. Plant Biotechnol J. 2015; 14(1):29-39. PMC: 6120502. DOI: 10.1111/pbi.12333. View

Lange B, Ghassemian M . Genome organization in Arabidopsis thaliana: a survey for genes involved in isoprenoid and chlorophyll metabolism. Plant Mol Biol. 2003; 51(6):925-48. DOI: 10.1023/a:1023005504702. View

Cho K, Han E, Kwak S, Cho J, Im J, Hong S . Expressing the sweet potato orange gene in transgenic potato improves drought tolerance and marketable tuber production. C R Biol. 2016; 339(5-6):207-213. DOI: 10.1016/j.crvi.2016.04.010. View

Yu Q, Beyer P . Reaction specificities of the ε-ionone-forming lycopene cyclase from rice (Oryza sativa) elucidated in vitro. FEBS Lett. 2012; 586(19):3415-20. DOI: 10.1016/j.febslet.2012.07.060. View

Harjes C, Rocheford T, Bai L, Brutnell T, Kandianis C, Sowinski S . Natural genetic variation in lycopene epsilon cyclase tapped for maize biofortification. Science. 2008; 319(5861):330-3. PMC: 2933658. DOI: 10.1126/science.1150255. View

Zhou X, McQuinn R, Fei Z, Wolters A, Van Eck J, Brown C . Regulatory control of high levels of carotenoid accumulation in potato tubers. Plant Cell Environ. 2011; 34(6):1020-1030. DOI: 10.1111/j.1365-3040.2011.02301.x. View

10.

Wang Z, Ke Q, Kim M, Kim S, Ji C, Jeong J . Transgenic alfalfa plants expressing the sweetpotato Orange gene exhibit enhanced abiotic stress tolerance. PLoS One. 2015; 10(5):e0126050. PMC: 4422619. DOI: 10.1371/journal.pone.0126050. View

11.

Fujisawa M, Nakano T, Shima Y, Ito Y . A large-scale identification of direct targets of the tomato MADS box transcription factor RIPENING INHIBITOR reveals the regulation of fruit ripening. Plant Cell. 2013; 25(2):371-86. PMC: 3608766. DOI: 10.1105/tpc.112.108118. View

12.

Kim S, Jeong J, Park S, Bae J, Ahn M, Lee H . Down-regulation of sweetpotato lycopene β-cyclase gene enhances tolerance to abiotic stress in transgenic calli. Mol Biol Rep. 2014; 41(12):8137-48. DOI: 10.1007/s11033-014-3714-4. View

13.

Walter M, Strack D . Carotenoids and their cleavage products: biosynthesis and functions. Nat Prod Rep. 2011; 28(4):663-92. DOI: 10.1039/c0np00036a. View

14.

Tanaka Y, Ohmiya A . Seeing is believing: engineering anthocyanin and carotenoid biosynthetic pathways. Curr Opin Biotechnol. 2008; 19(2):190-7. DOI: 10.1016/j.copbio.2008.02.015. View

15.

DallOsto L, Fiore A, Cazzaniga S, Giuliano G, Bassi R . Different roles of alpha- and beta-branch xanthophylls in photosystem assembly and photoprotection. J Biol Chem. 2007; 282(48):35056-68. DOI: 10.1074/jbc.M704729200. View

16.

Lu S, Van Eck J, Zhou X, Lopez A, OHalloran D, Cosman K . The cauliflower Or gene encodes a DnaJ cysteine-rich domain-containing protein that mediates high levels of beta-carotene accumulation. Plant Cell. 2006; 18(12):3594-605. PMC: 1785402. DOI: 10.1105/tpc.106.046417. View

17.

Okada K, Saito T, Nakagawa T, Kawamukai M, Kamiya Y . Five geranylgeranyl diphosphate synthases expressed in different organs are localized into three subcellular compartments in Arabidopsis. Plant Physiol. 2000; 122(4):1045-56. PMC: 58939. DOI: 10.1104/pp.122.4.1045. View

18.

Chen W, He S, Liu D, Patil G, Zhai H, Wang F . A Sweetpotato Geranylgeranyl Pyrophosphate Synthase Gene, IbGGPS, Increases Carotenoid Content and Enhances Osmotic Stress Tolerance in Arabidopsis thaliana. PLoS One. 2015; 10(9):e0137623. PMC: 4574098. DOI: 10.1371/journal.pone.0137623. View

19.

Lopez A, Van Eck J, Conlin B, Paolillo D, ONeill J, Li L . Effect of the cauliflower Or transgene on carotenoid accumulation and chromoplast formation in transgenic potato tubers. J Exp Bot. 2008; 59(2):213-23. DOI: 10.1093/jxb/erm299. View

20.

Kim S, Ahn Y, Ahn M, Lee H, Kwak S . Down-regulation of β-carotene hydroxylase increases β-carotene and total carotenoids enhancing salt stress tolerance in transgenic cultured cells of sweetpotato. Phytochemistry. 2011; 74:69-78. DOI: 10.1016/j.phytochem.2011.11.003. View