Plant Genome Editing with TALEN and CRISPR

Overview

Journal Cell Biosci

Publisher Biomed Central

Specialty Biology

Date 2017 Apr 29

PMID 28451378

Citations 81

Authors

Aimee Malzahn

Levi Lowder

Yiping Qi

Affiliations

Soon will be listed here.

Abstract

Genome editing promises giant leaps forward in advancing biotechnology, agriculture, and basic research. The process relies on the use of sequence specific nucleases (SSNs) to make DNA double stranded breaks at user defined genomic loci, which are subsequently repaired by two main DNA repair pathways: non-homologous end joining (NHEJ) and homology directed repair (HDR). NHEJ can result in frameshift mutations that often create genetic knockouts. These knockout lines are useful for functional and reverse genetic studies but also have applications in agriculture. HDR has a variety of applications as it can be used for gene replacement, gene stacking, and for creating various fusion proteins. In recent years, transcription activator-like effector nucleases and clustered regularly interspaced palindromic repeats (CRISPR) and CRISPR associated protein 9 or CRISPR from Prevotella and Francisella 1 have emerged as the preferred SSNs for research purposes. Here, we review their applications in plant research, discuss current limitations, and predict future research directions in plant genome editing.

Citing Articles

CRISPR-Cas applications in agriculture and plant research.

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Identification, charectrization and genetic transformation of lignin and pectin polysaccharides through CRISPR/Cas9 in Nicotiana tobacum.

Ahmed R, Ren A, Alshaya D, Fiaz S, Kong Y, Liaqat S Funct Integr Genomics. 2024; 24(5):188.

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Genetic engineering and genome editing technologies as catalyst for Africa's food security: the case of plant biotechnology in Nigeria.

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