Development of Molecular Markers for Iron Metabolism Related Genes in Lentil and Their Expression Analysis Under Excess Iron Stress

Overview

Journal Front Plant Sci

Date 2017 Apr 29

PMID 28450880

Citations 4

Authors

Debjyoti Sen Gupta

Kevin McPhee

Shiv Kumar

Affiliations

Soon will be listed here.

Abstract

Multiple genes and transcription factors are involved in the uptake and translocation of iron in plants from soil. The sequence information about iron uptake and translocation related genes is largely unknown in lentil ( Medik.). This study was designed to develop iron metabolism related molecular markers for (Basic helix loop helix), or FER-like transcription factor protein and (Iron related transporter) genes using genome synteny with barrel medic (). The second objective of this study was to analyze differential gene expression under excess iron over time (2 h, 8 h, 24 h). Specific molecular markers were developed for iron metabolism related genes () and validated in lentil. Gene specific markers for and were used for quantitative PCR (qPCR) studies based on their amplification efficiency. Significant differential expression of and was observed under excess iron conditions through qPCR based gene expression analysis. Regulation of iron uptake and translocation in lentil needs further characterization. Greater emphasis should be given to development of conditions simulating field conditions under external iron supply and considering adult plant physiology.

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