Data of Sperm-entry Inability in Ovarian Follicles That Are Depleted of S36 Chorionic Protein
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This paper presents data associated with the research article entitled "Targeted downregulation of s36 protein unearths its cardinal role in chorion biogenesis and architecture during oogenesis" [1]. chorion is produced by epithelial follicle cells and one of its functional serving role is egg fertilization through the micropyle, a specialized narrow channel at the anterior tip of the egg [2]. Sperm entry during fertilization is necessary for the egg to complete meiosis [3]. flies being characterized by severe downregulation of the s36 chorionic protein, specifically in the follicle-cell compartment of their ovary, appear with impaired fly fertility (Velentzas et al., 2016) [1]. In an effort to further investigate whether the observed infertility in the s36-targeted flies derives from a fertilization failure, such as the inability of sperm to pass through egg׳s micropyle, we mated females carrying s36-depleted ovaries with males expressing the GFP protein either in their sperm tails, or in both their sperm tails and sperm heads.
Velentzas A, Velentzas P, Katarachia S, Anagnostopoulos A, Sagioglou N, Thanou E Sci Rep. 2018; 8(1):16103.
PMID: 30382186 PMC: 6208399. DOI: 10.1038/s41598-018-34532-2.