Self-(in)compatibility in Apricot Germplasm is Controlled by Two Major Loci, S and M

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2017 Apr 27

PMID 28441955

Citations 9

Authors

Juan Vicente Munoz-Sanz

Elena Zuriaga

Inmaculada Lopez

Maria L Badenes

Carlos Romero

Affiliations

Soon will be listed here.

Abstract

Background: Apricot (Prunus armeniaca L.) exhibits a gametophytic self-incompatibility (GSI) system and it is mostly considered as a self-incompatible species though numerous self-compatible exceptions occur. These are mainly linked to the mutated S -haplotype carrying an insertion in the S-locus F-box gene that leads to a truncated protein. However, two S-locus unlinked pollen-part mutations (PPMs) termed m and m' have also been reported to confer self-compatibility (SC) in the apricot cultivars 'Canino' and 'Katy', respectively. This work was aimed to explore whether other additional mutations might explain SC in apricot as well.

Results: A set of 67 cultivars/accessions with different geographic origins were analyzed by PCR-screening of the S- and M-loci genotypes, contrasting results with the available phenotype data. Up to 20 S-alleles, including 3 new ones, were detected and sequence analysis revealed interesting synonymies and homonymies in particular with S-alleles found in Chinese cultivars. Haplotype analysis performed by genotyping and determining linkage-phases of 7 SSR markers, showed that the m and m' PPMs are linked to the same m haplotype. Results indicate that m -haplotype is tightly associated with SC in apricot germplasm being quite frequent in Europe and North-America. However, its prevalence is lower than that for S in terms of frequency and geographic distribution. Structures of 34 additional M-haplotypes were inferred and analyzed to depict phylogenetic relationships and M was found to be the closest haplotype to m Genotyping results showed that four cultivars classified as self-compatible do not have neither the S - nor the m -haplotype.

Conclusions: According to apricot germplasm S-genotyping, a loss of genetic diversity affecting the S-locus has been produced probably due to crop dissemination. Genotyping and phenotyping data support that self-(in)compatibility in apricot relies mainly on the S- but also on the M-locus. Regarding this latter, we have shown that the m -haplotype associated with SC is shared by 'Canino', 'Katy' and many other cultivars. Its origin is still unknown but phylogenetic analysis supports that m arose later in time than S from a widely distributed M-haplotype. Lastly, other mutants putatively carrying new mutations conferring SC have also been identified deserving future research.

Citing Articles

Genetic dissection of fruit maturity date in apricot (P. armeniaca L.) through a Single Primer Enrichment Technology (SPET) approach.

Baccichet I, Chiozzotto R, Scaglione D, Bassi D, Rossini L, Cirilli M BMC Genomics. 2022; 23(1):712.

PMID: 36258163 PMC: 9580121. DOI: 10.1186/s12864-022-08901-1.

Self-Incompatibility in Apricot: Identifying Pollination Requirements to Optimize Fruit Production.

Herrera S, Lora J, Hormaza J, Rodrigo J Plants (Basel). 2022; 11(15).

PMID: 35956497 PMC: 9370128. DOI: 10.3390/plants11152019.

Development of an HRMA-Based Marker Assisted Selection (MAS) Approach for Cost-Effective Genotyping of and Loci Controlling Self-Compatibility in Apricot ( L.).

Orlando Marchesano B, Chiozzotto R, Baccichet I, Bassi D, Cirilli M Genes (Basel). 2022; 13(3).

PMID: 35328101 PMC: 8954599. DOI: 10.3390/genes13030548.

LC-MS based metabolic fingerprinting of apricot pistils after self-compatible and self-incompatible pollinations.

Lenart J, Gere A, Causon T, Hann S, Dernovics M, Nemeth O Plant Mol Biol. 2020; 105(4-5):435-447.

PMID: 33296063 PMC: 7892686. DOI: 10.1007/s11103-020-01098-5.

Adoption and Optimization of Genomic Selection To Sustain Breeding for Apricot Fruit Quality.

Nsibi M, Gouble B, Bureau S, Flutre T, Sauvage C, Audergon J G3 (Bethesda). 2020; 10(12):4513-4529.

PMID: 33067307 PMC: 7718743. DOI: 10.1534/g3.120.401452.

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