Quality and Freshness of Human Bone Marrow-derived Mesenchymal Stem Cells Decrease over Time After Trypsinization and Storage in Phosphate-buffered Saline

Overview

Journal Sci Rep

Specialty Science

Date 2017 Apr 26

PMID 28439086

Citations 16

Authors

Tae Hwan Shin

Seungah Lee

Ki Ryung Choi

Da Yeon Lee

Yongman Kim

Man Jeong Paik

Chan Seo

Seok Kang

Moon Suk Jin

Tae Hyeon Yoo

Seong Ho Kang

Gwang Lee

Affiliations

Soon will be listed here.

Abstract

Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) have been studied for their therapeutic potential. However, evaluating the quality of hBM-MSCs before transplantation remains a challenge. We addressed this issue in the present study by investigating deformation, the expression of genes related to reactive oxygen species (ROS) generation, changes in amino acid profiles, and membrane fluidity in hBM-MSCs. Deformability and cell size were decreased after storage for 6 and 12 h, respectively, in phosphate-buffered saline. Intracellular ROS levels also increased over time, which was associated with altered expression of genes related to ROS generation and amino acid metabolism. Membrane fluidity measurements revealed higher Laurdan generalized polarization values at 6 and 12 h; however, this effect was reversed by N-acetyl-L-cysteine-treatment. These findings indicate that the quality and freshness of hBM-MSCs is lost over time after dissociation from the culture dish for transplantation, highlighting the importance of using freshly trypsinized cells in clinical applications.

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