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A Simple and Highly Efficient -mediated Transformation Protocol for

Overview
Journal Biotechnol Rep (Amst)
Specialty Biotechnology
Date 2017 Apr 25
PMID 28435807
Citations 22
Authors
Polyana Kelly Martins
Ana Paula Ribeiro
Barbara Andrade Dias Brito da Cunha
Adilson Kenji Kobayashi
Hugo Bruno Correa Molinari
Affiliations
Soon will be listed here.
Abstract

The production and use of sugarcane in Brazil is very important for bioenergy production and is recognized as one of the most efficient in the world. In our laboratory, is being tested as a model plant for sugarcane. has biological attributes (rapid life cycle, small genome, diploid, short stature and simple growth requirements) that make it suitable for use as a model system. We report a highly efficient protocol for -mediated genetic transformation of . The optimization of several steps in tissue culture allowed the rapid regeneration of plants and increased the rate of transformation up to 29%. This protocol could become a powerful tool for functional genomics in sugarcane.

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