The Sources of Calcium for Noradrenaline-induced Contraction in the Human Thoracic Internal Artery

Overview

Journal Pflugers Arch

Specialty Physiology

Date 2017 Apr 24

PMID 28434061

Authors

Leszek Buzun

Beata Modzelewska

Anna Kostrzewska

Ewa Kleszczewska

Tomasz Kleszczewski

Affiliations

Soon will be listed here.

Abstract

The aim of the present study was to examine the contribution of intracellular and extracellular calcium sources in contraction caused by noradrenaline (NA) of the human internal thoracic artery (ITA) in vitro. Distal segments of ITA were obtained from 20 patients (aged 38-73, at the time of routine coronary artery surgical revascularization (CABG)). Contractile responses to 10 mol/L NA in the physiological salt solution and in Ca-free solution without and after incubation with 10 mol/L thapsigargin (TSG) were recorded under isometric conditions. Responses of ITA rings to 1 μM NA without incubation with TSG accounted (% of reaction to 80 mM KCl) 224.70 ± 14.06% in PSS solution, 141.30 ± 8.66% in Ca-free solution, and 80.03 ± 1.71% after PSS restoration and were statistically significantly different (p < 0.0001, one-way ANOVA). Responses of ITA rings to 1 μM NA with 1 μM TSG accounted (% of reaction to 80 mM KCl) 114.50 ± 2.79% in Ca-free solution and 36.70 ± 2.38% after PSS restoration. Responses in Ca-free solution and after PSS restoration without and with TSG were statistically significantly different (p = 0.0257 and p < 0.0001, respectively-t test). ITA contraction is caused by calcium derived not only from the SR and the extracellular matrix. The delivery of calcium to the space surrounding tissue does not immediately deliver calcium to the myofilaments.

References

Palao T, Rippe C, van Veen H, VanBavel E, Sward K, Bakker E . Thrombospondin-4 knockout in hypertension protects small-artery endothelial function but induces aortic aneurysms. Am J Physiol Heart Circ Physiol. 2016; 310(11):H1486-93. DOI: 10.1152/ajpheart.00046.2016. View

Smith J . Calcium homeostasis in smooth muscle cells. New Horiz. 1996; 4(1):2-18. View

Zhou H, Kong D, Pan Q, Wang H . Sources of calcium in agonist-induced contraction of rat distal colon smooth muscle in vitro. World J Gastroenterol. 2008; 14(7):1077-83. PMC: 2689411. DOI: 10.3748/wjg.14.1077. View

Rabbani G, Vijay V, Sarabu M, Gupte S . Regulation of human internal mammary and radial artery contraction by extracellular and intracellular calcium channels and cyclic adenosine 3', 5' monophosphate. Ann Thorac Surg. 2007; 83(2):510-5. DOI: 10.1016/j.athoracsur.2006.09.027. View

He G, Yang C, Starr A . Overview of the nature of vasoconstriction in arterial grafts for coronary operations. Ann Thorac Surg. 1995; 59(3):676-83. DOI: 10.1016/0003-4975(94)01011-0. View

Rizzuto R, Marchi S, Bonora M, Aguiari P, Bononi A, De Stefani D . Ca(2+) transfer from the ER to mitochondria: when, how and why. Biochim Biophys Acta. 2009; 1787(11):1342-51. PMC: 2730423. DOI: 10.1016/j.bbabio.2009.03.015. View

Capiod T . Cell proliferation, calcium influx and calcium channels. Biochimie. 2011; 93(12):2075-9. DOI: 10.1016/j.biochi.2011.07.015. View

Jett G, Arcici Jr J, HATCHER Jr C, Abel P, Guyton R . Vasodilator drug effects on internal mammary artery and saphenous vein grafts. J Am Coll Cardiol. 1988; 11(6):1317-24. DOI: 10.1016/0735-1097(88)90298-7. View

Jett G, Arcidi Jr J, Dorsey L, HATCHER Jr C, Guyton R . Vasoactive drug effects on blood flow in internal mammary artery and saphenous vein grafts. J Thorac Cardiovasc Surg. 1987; 94(1):2-11. View

10.

Vidal C, Grassin-Delyle S, Devillier P, Naline E, Lansac E, Menasche P . Effect of severe acidosis on vasoactive effects of epinephrine and norepinephrine in human distal mammary artery. J Thorac Cardiovasc Surg. 2013; 147(5):1698-705. DOI: 10.1016/j.jtcvs.2013.11.013. View

11.

Kostrzewska A, Modzelewska B, Batra S . Source of calcium for contractile responses of large and small human intramyometrial arteries. Hum Reprod. 2000; 15(9):1927-31. DOI: 10.1093/humrep/15.9.1927. View

12.

Giessler C, Wangemann T, Silber R, Dhein S, Brodde O . Noradrenaline-induced contraction of human saphenous vein and human internal mammary artery: involvement of different alpha-adrenoceptor subtypes. Naunyn Schmiedebergs Arch Pharmacol. 2002; 366(2):104-9. DOI: 10.1007/s00210-002-0582-6. View

13.

Treiman M, Caspersen C, Christensen S . A tool coming of age: thapsigargin as an inhibitor of sarco-endoplasmic reticulum Ca(2+)-ATPases. Trends Pharmacol Sci. 1998; 19(4):131-5. DOI: 10.1016/s0165-6147(98)01184-5. View

14.

Mustonen E, Ruskoaho H, Rysa J . Thrombospondins, potential drug targets for cardiovascular diseases. Basic Clin Pharmacol Toxicol. 2012; 112(1):4-12. DOI: 10.1111/bcpt.12026. View

15.

Parekh A, Brading A . The sources of calcium for carbachol-induced contraction in the circular smooth muscle of guinea-pig stomach. Br J Pharmacol. 1991; 104(2):412-8. PMC: 1908568. DOI: 10.1111/j.1476-5381.1991.tb12444.x. View

16.

He G, Acuff T, Ryan W, Yang C, Douthit M, Bowman R . Inhibitory effects of calcium antagonists on alpha-adrenoceptor-mediated contraction in the human internal mammary artery. Br J Clin Pharmacol. 1994; 37(2):173-9. PMC: 1364594. DOI: 10.1111/j.1365-2125.1994.tb04257.x. View

17.

Laporte R, Hui A, Laher I . Pharmacological modulation of sarcoplasmic reticulum function in smooth muscle. Pharmacol Rev. 2004; 56(4):439-513. DOI: 10.1124/pr.56.4.1. View

18.

Sanders K . Invited review: mechanisms of calcium handling in smooth muscles. J Appl Physiol (1985). 2001; 91(3):1438-49. DOI: 10.1152/jappl.2001.91.3.1438. View

19.

Blaustein M, Golovina V, Song H, Choate J, Lencesova L, Robinson S . Organization of Ca2+ stores in vascular smooth muscle: functional implications. Novartis Found Symp. 2002; 246:125-37; discussion 137-41, 221-7. View

20.

Thastrup O, Cullen P, Drobak B, Hanley M, Dawson A . Thapsigargin, a tumor promoter, discharges intracellular Ca2+ stores by specific inhibition of the endoplasmic reticulum Ca2(+)-ATPase. Proc Natl Acad Sci U S A. 1990; 87(7):2466-70. PMC: 53710. DOI: 10.1073/pnas.87.7.2466. View