Regulation of Viral Gene Expression by Duck Enteritis Virus UL54

Overview

Journal Sci Rep

Specialty Science

Date 2017 Apr 23

PMID 28432334

Citations 9

Authors

Chaoyue Liu

Anchun Cheng

Mingshu Wang

Shun Chen

Renyong Jia

Dekang Zhu

Mafeng Liu

Kunfeng Sun

Qiao Yang

Ying Wu

Xinxin Zhao

Xiaoyue Chen

Affiliations

Soon will be listed here.

Abstract

Duck enteritis virus (DEV) UL54 is a homologue of human herpes simplex virus-1 (HSV-1) ICP27, which plays essential regulatory roles during infection. Our previous studies indicated that DEV UL54 is an immediate-early protein that can shuttle between the nucleus and the cytoplasm. In the present study, we found that UL54-deleted DEV (DEV-ΔUL54) exhibits growth kinetics, a plaque size and a viral DNA copy number that are significantly different from those of its parent wild-type virus (DEV-LoxP) and the revertant (DEV-ΔUL54 (Revertant)). Relative viral mRNA levels, reflecting gene expression, the transcription phase and the translation stage, are also significantly different between DEV-ΔUL54-infected cells and DEV-LoxP/DEV-ΔUL54 (Revertant)-infected cells. However, the localization pattern of UL30 mRNA is obviously changed in DEV-ΔUL54-infected cells. These findings suggest that DEV UL54 is important for virus growth and may regulate viral gene expression during transcription, mRNA export and translation.

Citing Articles

A Recombinant Duck Plague Virus Containing the ICP27 Deletion Marker Provides Robust Protection in Ducks.

Wu Y, Liu L, Zhang M, Zhan H, Wang C, Wang M Microbiol Spectr. 2023; 11(4):e0098323.

PMID: 37404171 PMC: 10434260. DOI: 10.1128/spectrum.00983-23.

Duck plague virus UL41 protein inhibits RIG-I/MDA5-mediated duck IFN-β production via mRNA degradation activity.

He T, Wang M, Cheng A, Yang Q, Wu Y, Jia R Vet Res. 2022; 53(1):22.

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The LORF5 Gene Is Non-essential for Replication but Important for Duck Plague Virus Cell-to-Cell Spread Efficiently in Host Cells.

Shen B, Li Y, Cheng A, Wang M, Wu Y, Yang Q Front Microbiol. 2021; 12:744408.

PMID: 34925260 PMC: 8674210. DOI: 10.3389/fmicb.2021.744408.

Duck Enteritis Virus VP16 Antagonizes IFN--Mediated Antiviral Innate Immunity.

Li Y, Wang M, Cheng A, Jia R, Yang Q, Chen S J Immunol Res. 2020; 2020:9630452.

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The Pivotal Roles of US3 Protein in Cell-to-Cell Spread and Virion Nuclear Egress of Duck Plague Virus.

Deng L, Wang M, Cheng A, Yang Q, Wu Y, Jia R Sci Rep. 2020; 10(1):7181.

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