Human MicroRNA-299-3p Decreases Invasive Behavior of Cancer Cells by Downregulation of Oct4 Expression and Causes Apoptosis

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2017 Apr 21

PMID 28426762

Citations 10

Authors

Axel R Gohring

Stefanie Reuter

Joachim H Clement

Xinlai Cheng

Jannick Theobald

Stefan Wolfl

Ralf Mrowka

Affiliations

Soon will be listed here.

Abstract

Purpose: Oct4 was reported to be one of the most important pluripotency transcription factors in the biology of stem cells including cancer stem cells, and progressed malignant cells. Here we report the investigation of gene expression control of Oct4 by selected human microRNAs and the physiological effect of Oct4 silencing in invasive cancer cells.

Methods And Results: High throughput luciferase activity assay revealed the microRNA-299-3p to be the most effective in reducing gene expression of Oct4, which was confirmed by Western blot analysis and Oct4 promoter activity in a target luciferase assay. Furthermore, it could be demonstrated that downregulation of Oct4 by microRNAs-299-3p in breast cancer and fibrosarcoma cells lead to a decreased invasiveness in a microfluidic chip assay. Additionally, microRNA-299-3p causes apoptosis in cancer cells. Comparison with Oct4 specific siRNA transfection confirmed that this effect is primary due to the blockade of Oct4 expression.

Conclusion: The results suggest that microRNA-299-3p is an interesting target for potential clinical use. It may be able to decrease invasive behaviour of carcinoma cells; or even kill these cells by causing apoptosis.

Citing Articles

Circular RNA CDR1as Mediated by Human Antigen R (HuR) Promotes Gastric Cancer Growth via miR-299-3p/TGIF1 Axis.

Li R, Xu X, Gao S, Wang J, Hou J, Xie Z Cancers (Basel). 2023; 15(23).

PMID: 38067260 PMC: 10705315. DOI: 10.3390/cancers15235556.

miR-299-3p suppresses cell progression and induces apoptosis by downregulating PAX3 in gastric cancer.

Wang Z, Liu Q, Huang P, Cai G Open Life Sci. 2021; 16(1):266-276.

PMID: 33817318 PMC: 8005920. DOI: 10.1515/biol-2021-0022.

Hsa_circ_0000231 knockdown inhibits the glycolysis and progression of colorectal cancer cells by regulating miR-502-5p/MYO6 axis.

Liu Y, Li H, Ye X, Ji A, Fu X, Wu H World J Surg Oncol. 2020; 18(1):255.

PMID: 32993655 PMC: 7526375. DOI: 10.1186/s12957-020-02033-0.

Epigenetic Features of Human Perinatal Stem Cells Redefine Their Stemness Potential.

Gaggi G, Di Credico A, Izzicupo P, Antonucci I, Crescioli C, di Giacomo V Cells. 2020; 9(5).

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The DNMT1/miR-34a/FOXM1 Axis Contributes to Stemness of Liver Cancer Cells.

Cao X, Liu L, Cao X, Cui Y, Zou C, Chen A J Oncol. 2020; 2020:8978930.

PMID: 32308683 PMC: 7142390. DOI: 10.1155/2020/8978930.

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