Influences of Realgar-, A Traditional Chinese Medicine Formula, on the Main CYP450 Activities in Rats Using a Cocktail Method

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2017 Apr 20

PMID 28421119

Citations 2

Authors

Huan-Hua Xu

Fei-Ran Hao

Mei-Xi Wang

Si-Jia Ren

Ming Li

Hong-Ling Tan

Yu-Guang Wang

Xiang-Lin Tang

Cheng-Rong Xiao

Qian-de Liang

Yue Gao

Zeng-Chun Ma

Affiliations

Soon will be listed here.

Abstract

The purpose of this work was to study the influences of Realgar- (RIF) and its principal element realgar on 4 main cytochrome P450 enzymes activities in rats. A simple and efficient cocktail method was developed to detect the four probe drugs simultaneously. In this study, Wistar rats were administered intragastric RIF and realgar for 14 days; mixed probe drugs were injected into rats by caudal vein. Through analyzing the pharmacokinetic parameter of mixed probe drugs in rats, we can calculate the CYPs activities. The results showed that RIF could inhibit CYP1A2 enzyme activity and induce CYP2C11 enzyme activity significantly. Interestingly, in realgar high dosage group, CYP3A1/2 enzyme activity was inhibited significantly, and different dosage of realgar manifested a good dose-dependent manner. The RIF results indicated that drug coadministrated with RIF may need to be paid attention in relation to drug-drug interactions (DDIs). Realgar, a toxic traditional Chinese medicine (TCM), does have curative effect on acute promyelocytic leukemia (APL). Its toxicity studies should be focused on. We found that, in realgar high dosage group, CYP3A1/2 enzymes activity was inhibited. This phenomenon may explain its potential toxicity mechanism.

Citing Articles

Pharmacology, Toxicology, and Rational Application of Cinnabar, Realgar, and Their Formulations.

Guan H, Xu Y, Ma C, Zhao D Evid Based Complement Alternat Med. 2022; 2022:6369150.

PMID: 36204126 PMC: 9532072. DOI: 10.1155/2022/6369150.

Safety and efficacy of Compound Huangdai Tablets combined with all-trans retinoic acid for treatment of acute promyelocytic leukemia: Clinical evidence and potential mechanisms.

Huang Q, Wang T, Xiong Y, Qu L, Yin Q, Zou W Chin Herb Med. 2022; 14(1):154-165.

PMID: 36120125 PMC: 9476755. DOI: 10.1016/j.chmed.2021.09.004.

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