Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2017 Apr 20

PMID 28420224

Citations 21

Authors

You-Young Jo

Seong-Gon Kim

Kwang-Jun Kwon

HaeYong Kweon

Weon-Sik Chae

Won-Geun Yang

Eun-Young Lee

Hyun Seok

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to evaluate the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites. Forty Sprague Dawley rats were used for the animal experiments. Central calvarial bone (diameter: 8.0 mm) defects were grafted with AL, AL/HA, or AL/HA/SF. New bone formation was evaluated by histomorphometric analysis. To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation. Additionally, osteogenic markers, such as fibroblast growth factor (FGF)-23, osteoprotegerin (OPG), and Runt-related transcription factor (Runx2) were evaluated by qPCR or IHC at eight weeks post implantation. The AL/HA/SF group showed significantly higher new bone formation than did the control group ( = 0.044) and the AL group ( = 0.035) at four weeks post implantation. Additionally, the AL/HA/SF group showed lower relative TNF-α mRNA levels and higher FGF-23 mRNA levels than the other groups did at eight weeks post implantation. IHC results demonstrated that the AL/HA/SF group had lower TNF-α expression and higher OPG and Runx2 expression at eight weeks post implantation. Additionally, no evidence of the inflammatory reaction or giant cell formation was observed around the residual graft material. We concluded that the AL/HA/SF composite could be effective as a scaffold for bone tissue engineering.

Citing Articles

Advances in the application and research of biomaterials in promoting bone repair and regeneration through immune modulation.

Liu L, Chen H, Zhao X, Han Q, Xu Y, Liu Y Mater Today Bio. 2025; 30():101410.

PMID: 39811613 PMC: 11731593. DOI: 10.1016/j.mtbio.2024.101410.

The Application of Regenerated Silk Fibroin in Tissue Repair.

Li Z, Tan G, Xie H, Lu S Materials (Basel). 2024; 17(16).

PMID: 39203101 PMC: 11355482. DOI: 10.3390/ma17163924.

Silk fibroin-based scaffolds for tissue engineering.

Ma L, Dong W, Lai E, Wang J Front Bioeng Biotechnol. 2024; 12:1381838.

PMID: 38737541 PMC: 11084674. DOI: 10.3389/fbioe.2024.1381838.

Degradable Polymeric Bio(nano)materials and Their Biomedical Applications: A Comprehensive Overview and Recent Updates.

Kuperkar K, Atanase L, Bahadur A, Crivei I, Bahadur P Polymers (Basel). 2024; 16(2).

PMID: 38257005 PMC: 10818796. DOI: 10.3390/polym16020206.

Advanced Applications of Silk-Based Hydrogels for Tissue Engineering: A Short Review.

Akdag Z, Ulag S, Kalaskar D, Duta L, Gunduz O Biomimetics (Basel). 2023; 8(8).

PMID: 38132551 PMC: 10742028. DOI: 10.3390/biomimetics8080612.

References

Liu W, Fan J, Xu D, Zhang J, Cui Z . Epigallocatechin-3-gallate protects against tumor necrosis factor alpha induced inhibition of osteogenesis of mesenchymal stem cells. Exp Biol Med (Maywood). 2016; 241(6):658-66. PMC: 4950329. DOI: 10.1177/1535370215624020. View

Roesler H . The history of some fundamental concepts in bone biomechanics. J Biomech. 1987; 20(11-12):1025-34. DOI: 10.1016/0021-9290(87)90020-0. View

Tortelli F, Cancedda R . Three-dimensional cultures of osteogenic and chondrogenic cells: a tissue engineering approach to mimic bone and cartilage in vitro. Eur Cell Mater. 2009; 17:1-14. DOI: 10.22203/ecm.v017a01. View

Wongputtaraksa T, Ratanavaraporn J, Pichyangkura R, Damrongsakkul S . Surface modification of Thai silk fibroin scaffolds with gelatin and chitooligosaccharide for enhanced osteogenic differentiation of bone marrow-derived mesenchymal stem cells. J Biomed Mater Res B Appl Biomater. 2012; 100(8):2307-15. DOI: 10.1002/jbm.b.32802. View

Kim S, Kim M, Kweon H, Jo Y, Lee K, Lee J . Comparison of unprocessed silk cocoon and silk cocoon middle layer membranes for guided bone regeneration. Maxillofac Plast Reconstr Surg. 2016; 38(1):11. PMC: 4770059. DOI: 10.1186/s40902-016-0057-1. View

Lee Y, Park S, Lee S, Boo Y, Choi J, Kim J . Ucma, a direct transcriptional target of Runx2 and Osterix, promotes osteoblast differentiation and nodule formation. Osteoarthritis Cartilage. 2015; 23(8):1421-31. DOI: 10.1016/j.joca.2015.03.035. View

Bhattacharjee P, Naskar D, Maiti T, Bhattacharya D, Das P, Nandi S . Potential of non-mulberry silk protein fibroin blended and grafted poly(Є-caprolactone) nanofibrous matrices for in vivo bone regeneration. Colloids Surf B Biointerfaces. 2016; 143:431-439. DOI: 10.1016/j.colsurfb.2016.03.058. View

Yamamoto R, Minamizaki T, Yoshiko Y, Yoshioka H, Tanne K, Aubin J . 1alpha,25-dihydroxyvitamin D3 acts predominately in mature osteoblasts under conditions of high extracellular phosphate to increase fibroblast growth factor 23 production in vitro. J Endocrinol. 2010; 206(3):279-86. PMC: 2917591. DOI: 10.1677/JOE-10-0058. View

Kim J, Choi J, Jeong J, Jang E, Kim A, Kim S . Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells. BMB Rep. 2010; 43(1):52-6. DOI: 10.5483/bmbrep.2010.43.1.052. View

10.

Luo Z, Yang Y, Deng Y, Sun Y, Yang H, Wei S . Peptide-incorporated 3D porous alginate scaffolds with enhanced osteogenesis for bone tissue engineering. Colloids Surf B Biointerfaces. 2016; 143:243-251. DOI: 10.1016/j.colsurfb.2016.03.047. View

11.

Sarker A, Amirian J, Min Y, Lee B . HAp granules encapsulated oxidized alginate-gelatin-biphasic calcium phosphate hydrogel for bone regeneration. Int J Biol Macromol. 2015; 81:898-911. DOI: 10.1016/j.ijbiomac.2015.09.029. View

12.

Lee S, Um I, Kim S, Cha M . Evaluation of bone formation and membrane degradation in guided bone regeneration using a 4-hexylresorcinol-incorporated silk fabric membrane. Maxillofac Plast Reconstr Surg. 2015; 37(1):32. PMC: 4686547. DOI: 10.1186/s40902-015-0034-0. View

13.

Zhong Q, Li W, Su X, Li G, Zhou Y, Kundu S . Degradation pattern of porous CaCO3 and hydroxyapatite microspheres in vitro and in vivo for potential application in bone tissue engineering. Colloids Surf B Biointerfaces. 2016; 143:56-63. DOI: 10.1016/j.colsurfb.2016.03.020. View

14.

Kim H, Che L, Ha Y, Ryu W . Mechanically-reinforced electrospun composite silk fibroin nanofibers containing hydroxyapatite nanoparticles. Mater Sci Eng C Mater Biol Appl. 2014; 40:324-35. DOI: 10.1016/j.msec.2014.04.012. View

15.

Thomas A, Harding K, Moore K . Alginates from wound dressings activate human macrophages to secrete tumour necrosis factor-alpha. Biomaterials. 2000; 21(17):1797-802. DOI: 10.1016/s0142-9612(00)00072-7. View

16.

Vimalraj S, Arumugam B, Miranda P, Selvamurugan N . Runx2: Structure, function, and phosphorylation in osteoblast differentiation. Int J Biol Macromol. 2015; 78:202-8. DOI: 10.1016/j.ijbiomac.2015.04.008. View

17.

Lee S, Kim S, Balazsi C, Chae W, Lee H . Comparative study of hydroxyapatite from eggshells and synthetic hydroxyapatite for bone regeneration. Oral Surg Oral Med Oral Pathol Oral Radiol. 2012; 113(3):348-55. DOI: 10.1016/j.tripleo.2011.03.033. View

18.

Mandal B, Kundu S . Calcium alginate beads embedded in silk fibroin as 3D dual drug releasing scaffolds. Biomaterials. 2009; 30(28):5170-7. DOI: 10.1016/j.biomaterials.2009.05.072. View

19.

Sadat-Shojai M, Khorasani M, Dinpanah-Khoshdargi E, Jamshidi A . Synthesis methods for nanosized hydroxyapatite with diverse structures. Acta Biomater. 2013; 9(8):7591-621. DOI: 10.1016/j.actbio.2013.04.012. View

20.

Inoue S, Tanaka K, Arisaka F, Kimura S, Ohtomo K, Mizuno S . Silk fibroin of Bombyx mori is secreted, assembling a high molecular mass elementary unit consisting of H-chain, L-chain, and P25, with a 6:6:1 molar ratio. J Biol Chem. 2000; 275(51):40517-28. DOI: 10.1074/jbc.M006897200. View