Transcription of Proto-oncogenes in Rous Sarcoma Virus Infected and Transformed Chicken Embryo Cells

Overview

Journal Arch Virol

Specialty Microbiology

Date 1988 Jan 1

PMID 2840872

Authors

C Bullacher

A Barnekow

Affiliations

Soon will be listed here.

Abstract

To evaluate an effect of infection by RSV on the transcriptional activity of proto-oncogenes, we tested 19 cellular oncogenes and the beta-actin gene performing "nuclear run on transcription assays" using nuclei isolated from RSV infected or transformed chick embryo cells (CEC). A drastic increase in the transcriptional activity of the c-fgr proto-oncogene and a slight increase of c-fos and c-myc could be observed in uninfected compared to RSV infected CEC. To distinguish between infection- and transformation-dependent alterations we used the transformation-defective temperature-sensitive RSV mutant strain NY 68. Ts NY 68 infected CEC maintained at the permissive temperature of 36 degrees C and exhibiting a transformed phenotype, displayed no significant differences in the transcriptional activity of the 19 proto-oncogenes when compared to ts NY 68 infected CEC maintained at the nonpermissive temperature of 42 degrees C and exhibiting a normal phenotype. We conclude that the alterations observed were caused by the viral infection and are not due to the process of transformation. No significant changes in the transcription rate of the investigated genes could be observed after shifting ts NY 68 infected CEC from 42 degrees C to 36 degrees C. In contrast, a shift of ts NY 68 infected CEC from 36 degrees C to 42 degrees C results, after 2 hours, in a transient increase of the c-fos transcription.

References

Naharro G, Tronick S, Rasheed S, Gardner M, Aaronson S, ROBBINS K . Molecular cloning of integrated Gardner-Rasheed feline sarcoma virus: genetic structure of its cell-derived sequence differs from that of other tyrosine kinase-coding onc genes. J Virol. 1983; 47(3):611-9. PMC: 255301. DOI: 10.1128/JVI.47.3.611-619.1983. View

DONNER L, Fedele L, Garon C, Anderson S, Sherr C . McDonough feline sarcoma virus: characterization of the molecularly cloned provirus and its feline oncogene (v-fms). J Virol. 1982; 41(2):489-500. PMC: 256777. DOI: 10.1128/JVI.41.2.489-500.1982. View

Rapp U, Goldsborough M, Mark G, Bonner T, Groffen J, Reynolds Jr F . Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus. Proc Natl Acad Sci U S A. 1983; 80(14):4218-22. PMC: 384008. DOI: 10.1073/pnas.80.14.4218. View

Bishop J . Viral oncogenes. Cell. 1985; 42(1):23-38. DOI: 10.1016/s0092-8674(85)80098-2. View

Lautenberger J, Schulz R, Garon C, Tsichlis P, Papas T . Molecular cloning of avian myelocytomatosis virus (MC29) transforming sequences. Proc Natl Acad Sci U S A. 1981; 78(3):1518-22. PMC: 319162. DOI: 10.1073/pnas.78.3.1518. View

Rabin M, Doherty P, Gottesman M . The tumor promoter phorbol 12-myristate 13-acetate induces a program of altered gene expression similar to that induced by platelet-derived growth factor and transforming oncogenes. Proc Natl Acad Sci U S A. 1986; 83(2):357-60. PMC: 322857. DOI: 10.1073/pnas.83.2.357. View

Carroll R, Ash J, Vogt P, Singer S . Reversion of transformed glycolysis to normal by inhibition of protein synthesis in rat kidney cells infected with temperature-sensitive mutant of Rous sarcoma virus. Proc Natl Acad Sci U S A. 1978; 75(10):5015-9. PMC: 336253. DOI: 10.1073/pnas.75.10.5015. View

Sherr C, Fedele L, Oskarsson M, Maizel J, Vande Woude G . Molecular cloning of Snyder-Theilen feline leukemia and sarcoma viruses: comparative studies of feline sarcoma virus with its natural helper virus and with Moloney murine sarcoma virus. J Virol. 1980; 34(1):200-12. PMC: 288686. DOI: 10.1128/JVI.34.1.200-212.1980. View

Westin E, Wong-Staal F, Gelmann E, Dalla-Favera R, Papas T, Lautenberger J . Expression of cellular homologues of retroviral onc genes in human hematopoietic cells. Proc Natl Acad Sci U S A. 1982; 79(8):2490-4. PMC: 346224. DOI: 10.1073/pnas.79.8.2490. View

10.

Kitamura N, Kitamura A, Toyoshima K, Hirayama Y, Yoshida M . Avian sarcoma virus Y73 genome sequence and structural similarity of its transforming gene product to that of Rous sarcoma virus. Nature. 1982; 297(5863):205-8. DOI: 10.1038/297205a0. View

11.

Hynes R . Fibronectins. Sci Am. 1986; 254(6):42-51. DOI: 10.1038/scientificamerican0686-42. View

12.

Nishimura S, Sekiya T . Human cancer and cellular oncogenes. Biochem J. 1987; 243(2):313-27. PMC: 1147857. DOI: 10.1042/bj2430313. View

13.

Gilmer T, Parsons J, Erikson R . Construction of plasmids for expression of Rous sarcoma virus transforming protein, p60src, in Escherichia coli. Proc Natl Acad Sci U S A. 1982; 79(7):2152-6. PMC: 346148. DOI: 10.1073/pnas.79.7.2152. View

14.

Gessler M, Barnekow A . Differential expression of the cellular oncogenes c-src and c-yes in embryonal and adult chicken tissues. Biosci Rep. 1984; 4(9):757-70. DOI: 10.1007/BF01128817. View

15.

Kuchino Y, Nemoto K, Kawai S, Nishimura S . Activation of c-myc gene transcription by Rous sarcoma virus infection. Jpn J Cancer Res. 1985; 76(2):75-8. View

16.

Perbal B, Baluda M . Avian myeloblastosis virus transforming gene is related to unique chicken DNA regions separated by at least one intervening sequence. J Virol. 1982; 41(1):250-7. PMC: 256746. DOI: 10.1128/JVI.41.1.250-257.1982. View

17.

Weinberg R . The action of oncogenes in the cytoplasm and nucleus. Science. 1985; 230(4727):770-6. DOI: 10.1126/science.2997917. View

18.

Cheah M, Ley T, Tronick S, ROBBINS K . fgr proto-oncogene mRNA induced in B lymphocytes by Epstein-Barr virus infection. Nature. 1986; 319(6050):238-40. DOI: 10.1038/319238a0. View

19.

Ruley H . Adenovirus early region 1A enables viral and cellular transforming genes to transform primary cells in culture. Nature. 1983; 304(5927):602-6. DOI: 10.1038/304602a0. View

20.

Spector D, Varmus H, Bishop J . Nucleotide sequences related to the transforming gene of avian sarcoma virus are present in DNA of uninfected vertebrates. Proc Natl Acad Sci U S A. 1978; 75(9):4102-6. PMC: 336059. DOI: 10.1073/pnas.75.9.4102. View