Differential Pattern of HIF-1α Expression in HNSCC Cancer Stem Cells After Carbon Ion or Photon Irradiation: One Molecular Explanation of the Oxygen Effect

Overview

Journal Br J Cancer

Specialty Oncology

Date 2017 Apr 14

PMID 28407653

Citations 34

Authors

Anne-Sophie Wozny

Alexandra Lauret

Priscillia Battiston-Montagne

Jean-Baptiste Guy

Michael Beuve

Micaela Cunha

Yannick Saintigny

Emilie Blond

Nicolas Magne

Philippe Lalle

Dominique Ardail

Gersende Alphonse

Claire Rodriguez-Lafrasse

Affiliations

Soon will be listed here.

Abstract

Background: Head and neck squamous cell carcinoma (HNSCC) are resistant to standard treatments, partly due to cancer stem cells (CSCs) localised in hypoxic niches. Compared to X-rays, carbon ion irradiation relies on better ballistic properties, higher relative biological effectiveness and the absence of oxygen effect. Hypoxia-inducible factor-1α (HIF-1α) is involved in the resistance to photons, whereas its role in response to carbon ions remains unclear.

Methods: Two HNSCC cell lines and their CSC sub-population were studied in response to photons or carbon ion irradiation, in normoxia or hypoxia, after inhibition or not of HIF-1α.

Results: Under hypoxia, compared to non-CSCs, HIF-1α is expressed earlier in CSCs. A combined effect photons/hypoxia, less observed with carbon ions, results in a synergic and earlier HIF-1α expression in both subpopulations. The diffuse ROS production by photons is concomitant with HIF-1α expression and essential to its activation. There is no oxygen effect in response to carbon ions and the ROS localised in the track might be insufficient to stabilise HIF-1α. Finally, in hypoxia, cells were sensitised to both types of radiations after HIF-1α inhibition.

Conclusions: Hypoxia-inducible factor-1α plays a main role in the response of CSCs and non-CSCs to carbon ion and photon irradiations, which makes the HIF-1α targeting an attractive therapeutic challenge.

Citing Articles

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A radiobiological perspective on radioresistance or/and radiosensitivity of head and neck squamous cell carcinoma.

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Hypoxia Modulates Radiosensitivity and Response to Different Radiation Qualities in A549 Non-Small Cell Lung Cancer (NSCLC) Cells.

Nisar H, Labonte F, Roggan M, Schmitz C, Chevalier F, Konda B Int J Mol Sci. 2024; 25(2).

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