Isolation and Characterization of the Genes for Two Small RNAs of Herpesvirus Papio and Their Comparison with Epstein-Barr Virus-encoded EBER RNAs

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1988 Aug 1

PMID 2839701

Citations 41

Authors

J G Howe

M D Shu

Affiliations

Soon will be listed here.

Abstract

Genes for the Epstein-Barr virus-encoded RNAs (EBERs), two low-molecular-weight RNAs encoded by the human gammaherpesvirus Epstein-Barr virus (EBV), hybridize to two small RNAs in a baboon cell line that contains a similar virus, herpesvirus papio (HVP). The genes for the HVP RNAs (HVP-1 and HVP-2) are located together in the small unique region at the left end of the viral genome and are transcribed by RNA polymerase III in a rightward direction, similar to the EBERs. There is significant similarity between EBER1 and HVP-1 RNA, except for an insert of 22 nucleotides which increases the length of HVP-1 RNA to 190 nucleotides. There is less similarity between the sequences of EBER2 and HVP-2 RNA, but both have a length of about 170 nucleotides. The predicted secondary structure of each HVP RNA is remarkably similar to that of the respective EBER, implying that the secondary structures are important for function. Upstream from the initiation sites of all four RNA genes are several highly conserved sequences which may function in the regulation of transcription. The HVP RNAs, together with the EBERs, are highly abundant in transformed cells and are efficiently bound by the cellular La protein.

Citing Articles

A Review of the Potential Role of Human Cytomegalovirus (HCMV) Infections in Breast Cancer Carcinogenesis and Abnormal Immunity.

Geisler J, Touma J, Rahbar A, Soderberg-Naucler C, Vetvik K Cancers (Basel). 2019; 11(12).

PMID: 31766600 PMC: 6966479. DOI: 10.3390/cancers11121842.

Tracking EBV-encoded RNAs (EBERs) from the nucleus to the excreted exosomes of B-lymphocytes.

Ahmed W, Tariq S, Khan G Sci Rep. 2018; 8(1):15438.

PMID: 30337610 PMC: 6193935. DOI: 10.1038/s41598-018-33758-4.

Epstein-Barr Virus EBER Transcripts Affect miRNA-Mediated Regulation of Specific Targets and Are Processed to Small RNA Species.

Alles J, Hasler D, Kazmi S, Tesson M, Hamilton A, Schlegel L Noncoding RNA. 2018; 1(3):170-191.

PMID: 29861423 PMC: 5932547. DOI: 10.3390/ncrna1030170.

miRNA expression profiling of Epstein-Barr virus-associated NKTL cell lines by Illumina deep sequencing.

Alles J, Menegatti J, Motsch N, Hart M, Eichner N, Reinhardt R FEBS Open Bio. 2016; 6(4):251-63.

PMID: 27239439 PMC: 4821355. DOI: 10.1002/2211-5463.12027.

Molecular screening for Epstein Barr virus (EBV) among Sudanese patients with nasopharyngeal carcinoma (NPC).

Ahmed H, Suliman R, El Aziz M, Alshammari F Infect Agent Cancer. 2015; 10:6.

PMID: 25705250 PMC: 4335634. DOI: 10.1186/s13027-015-0002-4.

References

Tinoco Jr I, Uhlenbeck O, Levine M . Estimation of secondary structure in ribonucleic acids. Nature. 1971; 230(5293):362-7. DOI: 10.1038/230362a0. View

Rawlins D, Milman G, Hayward S, Hayward G . Sequence-specific DNA binding of the Epstein-Barr virus nuclear antigen (EBNA-1) to clustered sites in the plasmid maintenance region. Cell. 1985; 42(3):859-68. DOI: 10.1016/0092-8674(85)90282-x. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Falk L, Deinhardt F, Nonoyama M, Wolfe L, Bergholz C . Properties of a baboon lymphotropic herpesvirus related to Epstein-Barr virus. Int J Cancer. 1976; 18(6):798-807. DOI: 10.1002/ijc.2910180611. View

Maxam A, Gilbert W . A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977; 74(2):560-4. PMC: 392330. DOI: 10.1073/pnas.74.2.560. View

Berk A, Sharp P . Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977; 12(3):721-32. DOI: 10.1016/0092-8674(77)90272-0. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

zur Hausen H, ONEILL F, Freese U, Hecker E . Persisting oncogenic herpesvirus induced by the tumour promotor TPA. Nature. 1978; 272(5651):373-5. DOI: 10.1038/272373a0. View

Sugden B, Phelps M, Domoradzki J . Epstein-Barr virus DNA is amplified in transformed lymphocytes. J Virol. 1979; 31(3):590-5. PMC: 353487. DOI: 10.1128/JVI.31.3.590-595.1979. View

10.

Brandsma J, Miller G . Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA. Proc Natl Acad Sci U S A. 1980; 77(11):6851-5. PMC: 350388. DOI: 10.1073/pnas.77.11.6851. View

11.

Zuker M, Stiegler P . Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information. Nucleic Acids Res. 1981; 9(1):133-48. PMC: 326673. DOI: 10.1093/nar/9.1.133. View

12.

Bhat R, Thimmappaya B . Construction and analysis of additional adenovirus substitution mutants confirm the complementation of VAI RNA function by two small RNAs encoded by Epstein-Barr virus. J Virol. 1985; 56(3):750-6. PMC: 252645. DOI: 10.1128/JVI.56.3.750-756.1985. View

13.

Wang D, Liebowitz D, Kieff E . An EBV membrane protein expressed in immortalized lymphocytes transforms established rodent cells. Cell. 1985; 43(3 Pt 2):831-40. DOI: 10.1016/0092-8674(85)90256-9. View

14.

OMalley R, Mariano T, Siekierka J, Mathews M . A mechanism for the control of protein synthesis by adenovirus VA RNAI. Cell. 1986; 44(3):391-400. DOI: 10.1016/0092-8674(86)90460-5. View

15.

Kallin B, Dillner J, Ernberg I, Rosen A, Henle W, Henle G . Four virally determined nuclear antigens are expressed in Epstein-Barr virus-transformed cells. Proc Natl Acad Sci U S A. 1986; 83(5):1499-503. PMC: 323104. DOI: 10.1073/pnas.83.5.1499. View

16.

Kitajewski J, Schneider R, Safer B, Munemitsu S, Samuel C, Thimmappaya B . Adenovirus VAI RNA antagonizes the antiviral action of interferon by preventing activation of the interferon-induced eIF-2 alpha kinase. Cell. 1986; 45(2):195-200. DOI: 10.1016/0092-8674(86)90383-1. View

17.

Larsson S, Svensson C, Akusjarvi G . Characterization of a low-molecular-weight virus-associated (VA) RNA encoded by simian adenovirus type 7 which functionally can substitute for adenovirus type 5 VA RNAI. J Virol. 1986; 60(2):635-44. PMC: 288936. DOI: 10.1128/JVI.60.2.635-644.1986. View

18.

Pesano R, Pagano J . Herpesvirus papio contains a plasmid origin of replication that acts in cis interspecies with an Epstein-Barr virus trans-acting function. J Virol. 1986; 60(3):1159-62. PMC: 253376. DOI: 10.1128/JVI.60.3.1159-1162.1986. View

19.

Howe J, Steitz J . Localization of Epstein-Barr virus-encoded small RNAs by in situ hybridization. Proc Natl Acad Sci U S A. 1986; 83(23):9006-10. PMC: 387063. DOI: 10.1073/pnas.83.23.9006. View

20.

BARK C, Weller P, Zabielski J, Janson L, Pettersson U . A distant enhancer element is required for polymerase III transcription of a U6 RNA gene. Nature. 1987; 328(6128):356-9. DOI: 10.1038/328356a0. View