[Quantity of Treg Cells and Th17 Cells in Spleen of Primary Immune Thrombocytopenic Purpura Patients]

To observe the quantity of Treg cells and Th17 cells in spleen of adult primary immune thrombocytopenic purpura (ITP) patients. 43 ITP cases with splenectomy treatment were enrolled from December 2008 to June 2016 at Union Hospital of Fujian Medical University, including 20 males and 23 females with a median age of 36 (18-76) years. The controls were thirty patients who underwent splenectomy because of pancreatic diseases or splenic impairment, including 21 males and 9 females with a median age of 47 (21-69) years. The quantity and ratio of Treg cells and Th17 cells were examined by immunohistochemistry between ITP patients and controls. ①The quantity of Treg cells in ITP were less than controls[ (11.3±4.7) /mm(2) (59.0±15.0) /mm(2), =-22.894, <0.001], but Th17 cells were more than controls[ (235.2±69.4) /mm(2) (181.1±23.7) /mm(2), =13.768, <0.001]. So the ratio of Treg/Th17 in ITP was lower than controls (0.048±0.027 0.328±0.086, =19.522, <0.001) . ② The quantity of Treg cells in cases without response after splenectomy were less than cases with response[ (9.5±5.0) /mm(2) (11.6±4.7) /mm(2), =2.723, =0.010], and there is no statistical differences between the two groups about the quantity of Th17 cells and the ratio of Treg/Th17 cells[ (232.3±80.8) /mm(2) (239.6±66.9) /mm(2), =1.108, =0.277; 0.040±0.024 0.053±0.027, =0.540, =0.592]. There is a significant difference about the quantity of Treg cells and Th17 cells in spleen between ITP patients and healthy controls, and they are relevant to the response after splenectomy.