Expression of Lens-related MicroRNAs in Transparent Infant Lenses and Congenital Cataract

Overview

Journal Int J Ophthalmol

Specialty Ophthalmology

Date 2017 Apr 11

PMID 28393025

Citations 4

Authors

Chang-Rui Wu

Min Ye

Li Qin

Yue Yin

Cheng Pei

Affiliations

Soon will be listed here.

Abstract

Aim: To identify the expression of lens-related microRNAs (miRNAs) in the central epithelium of transparent infant lenses and congenital cataract.

Methods: Lens-related miRNAs were retrieved from PubMed database. The expression levels of these miRNAs in transparent infant lenses and congenital cataract were determined by stem-loop reverse transcription-polymerase chain reaction (RT-PCR). miRanda algorithm was used to predict the target genes of these differentially expressed miRNAs. The target mRNA was validated.

Results: Six lens-related miRNAs were retrieved from screening PubMed database. The most abundant miRNA in transparent infant lenses according to stem-loop RT-PCR was miR-184. miR-182 was up-regulated in congenital cataract. Contrarily, miR-204 and miR-124 was down-regulated. miR-204 exhibited a more significant decrease in expression than miR-124. In addition, Meis2 was predicted to be the target of miR-204 using miRanda algorithm. miR-204 mimic/antagomir transfection experiments suggested the negative correlation between the expression of miR-204 and Meis2.

Conclusion: The expression levels of miR-182, miR-204 and miR-124 differ between the central epithelium of transparent infant lens and congenital cataract, suggesting their involvement in the pathogenesis of congenital cataract. miR-204 may act silencing Meis2 to regulate lens development and congenital cataract formation.

Citing Articles

Genome-Wide Analysis of Differentially Expressed miRNAs and Their Associated Regulatory Networks in Lenses Deficient for the Congenital Cataract-Linked Tudor Domain Containing Protein TDRD7.

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MicroRNA-182-5p protects human lens epithelial cells against oxidative stress-induced apoptosis by inhibiting NOX4 and p38 MAPK signalling.

Li Z, Ge M, Yuan Z BMC Ophthalmol. 2020; 20(1):233.

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Transcriptome analysis of neural progenitor cells derived from Lowe syndrome induced pluripotent stem cells: identification of candidate genes for the neurodevelopmental and eye manifestations.

Liu H, Barnes J, Pedrosa E, Herman N, Salas F, Wang P J Neurodev Disord. 2020; 12(1):14.

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TRPM3_miR-204: a complex locus for eye development and disease.

Shiels A Hum Genomics. 2020; 14(1):7.

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