Sodium Tanshinone IIA Sulfonate Prevents Radiation-Induced Toxicity in H9c2 Cardiomyocytes

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2017 Apr 8

PMID 28386289

Citations 8

Authors

Wenjing Zhang

Yi Li

Rui Li

Yaya Wang

Mengwen Zhu

Bowen Wang

Yanling Li

Dongyun Li

Ping Xie

Bin Liu

Affiliations

Soon will be listed here.

Abstract

The present study was designed to elucidate the key parameters associated with X-ray radiation induced oxidative stress and the effects of STS on X-ray-induced toxicity in H9c2 cardiomyocytes. Cytotoxicity of STS and radiation was assessed by MTT. Antioxidant activity was evaluated by SOD and MDA. Apoptosis was measured by the flow cytometry, Hoechst 33258, clonogenic survival assay, and western blot. It was found that the cell viability of H9c2 cells exposed to X-ray radiation was significantly decreased in a dose-dependent manner and was associated with cell cycle arrest at the G0/G1 phase as well as apoptosis. STS treatment significantly reversed the morphological changes, attenuated radiation-induced apoptosis, and improved the antioxidant activity in the H9c2 cells. STS significantly increased the Bcl-2 and Bcl-2/Bax levels and decreased the Bax and caspase-3 levels, compared with the cells treated with radiation alone. STS treatment also resulted in a significant increase in p38-MAPK activation. STS could protect the cells from X-ray-induced cell cycle arrest, oxidative stress, and apoptosis. Therefore, we suggest the STS could be useful for the treatment of radiation-induced cardiovascular injury.

Citing Articles

Role and molecular mechanism of traditional Chinese medicine in preventing cardiotoxicity associated with chemoradiotherapy.

Lv X, Wen R, Liu K, Zhao X, Pan C, Gao X Front Cardiovasc Med. 2022; 9:1047700.

PMID: 36419486 PMC: 9678083. DOI: 10.3389/fcvm.2022.1047700.

Tanshinone IIA Accomplished Protection against Radiation-Induced Cardiomyocyte Injury by Regulating the p38/p53 Pathway.

Wang G, Ma L, Wang B, Gao F, Li J, Cai H Mediators Inflamm. 2022; 2022:1478181.

PMID: 36046762 PMC: 9424041. DOI: 10.1155/2022/1478181.

Sodium Tanshinone II Sulfonate A Ameliorates Hypoxia-Induced Pulmonary Hypertension.

Bao Y, Chen J, Jiang Y, Wang L, Xue R, Qian J Front Pharmacol. 2020; 11:687.

PMID: 32508639 PMC: 7253651. DOI: 10.3389/fphar.2020.00687.

Protective effect of bacterial lipase on lipopolysaccharide-induced toxicity in rat cardiomyocytes; H9C2 cell line.

Mamipour M, Yousefi M, Dehnad A, Faridvand Y, Zarezadeh R, Khaksar M J Cardiovasc Thorac Res. 2020; 12(1):35-42.

PMID: 32211136 PMC: 7080329. DOI: 10.34172/jcvtr.2020.06.

in Treating Cardiovascular Diseases: A Review on Its Pharmacological and Clinical Applications.

Ren J, Fu L, Nile S, Zhang J, Kai G Front Pharmacol. 2019; 10:753.

PMID: 31338034 PMC: 6626924. DOI: 10.3389/fphar.2019.00753.

References

Armstrong G, Kawashima T, Leisenring W, Stratton K, Stovall M, Hudson M . Aging and risk of severe, disabling, life-threatening, and fatal events in the childhood cancer survivor study. J Clin Oncol. 2014; 32(12):1218-27. PMC: 3986385. DOI: 10.1200/JCO.2013.51.1055. View

Yang R, Liu A, Ma X, Li L, Su D, Liu J . Sodium tanshinone IIA sulfonate protects cardiomyocytes against oxidative stress-mediated apoptosis through inhibiting JNK activation. J Cardiovasc Pharmacol. 2008; 51(4):396-401. DOI: 10.1097/FJC.0b013e3181671439. View

Xu H, Chen K . Integrating traditional medicine with biomedicine towards a patient-centered healthcare system. Chin J Integr Med. 2011; 17(2):83-4. DOI: 10.1007/s11655-011-0641-2. View

Harake D, Franco V, Henkel J, Miller T, Lipshultz S . Cardiotoxicity in childhood cancer survivors: strategies for prevention and management. Future Cardiol. 2012; 8(4):647-70. PMC: 3870660. DOI: 10.2217/fca.12.44. View

Lipshultz S, Lipsitz S, Sallan S, Simbre 2nd V, Shaikh S, Mone S . Long-term enalapril therapy for left ventricular dysfunction in doxorubicin-treated survivors of childhood cancer. J Clin Oncol. 2002; 20(23):4517-22. DOI: 10.1200/JCO.2002.12.102. View

Gomez-Fernandez J . Functions of the C-terminal domains of apoptosis-related proteins of the Bcl-2 family. Chem Phys Lipids. 2014; 183:77-90. DOI: 10.1016/j.chemphyslip.2014.05.003. View

Ilavenil S, Kim D, Jeong Y, Valan Arasu M, Vijayakumar M, Prabhu P . Trigonelline protects the cardiocyte from hydrogen peroxide induced apoptosis in H9c2 cells. Asian Pac J Trop Med. 2015; 8(4):263-8. DOI: 10.1016/S1995-7645(14)60328-X. View

Fu J, Huang H, Liu J, Pi R, Chen J, Liu P . Tanshinone IIA protects cardiac myocytes against oxidative stress-triggered damage and apoptosis. Eur J Pharmacol. 2007; 568(1-3):213-21. DOI: 10.1016/j.ejphar.2007.04.031. View

Wang Y, Huang S, Sah V, ROSS Jr J, Brown J, Han J . Cardiac muscle cell hypertrophy and apoptosis induced by distinct members of the p38 mitogen-activated protein kinase family. J Biol Chem. 1998; 273(4):2161-8. DOI: 10.1074/jbc.273.4.2161. View

10.

Hudson M, Ness K, Gurney J, Mulrooney D, Chemaitilly W, Krull K . Clinical ascertainment of health outcomes among adults treated for childhood cancer. JAMA. 2013; 309(22):2371-2381. PMC: 3771083. DOI: 10.1001/jama.2013.6296. View

11.

Martin E, Olson A, Steeg C, Casarella W . Radiation exposure to the pediatric patient during cardiac catheterization and angiocardiography. Emphasis on the thyroid gland. Circulation. 1981; 64(1):153-8. DOI: 10.1161/01.cir.64.1.153. View

12.

Renault T, Teijido O, Antonsson B, Dejean L, Manon S . Regulation of Bax mitochondrial localization by Bcl-2 and Bcl-x(L): keep your friends close but your enemies closer. Int J Biochem Cell Biol. 2012; 45(1):64-7. DOI: 10.1016/j.biocel.2012.09.022. View

13.

Mezzaroma E, Di X, Graves P, Toldo S, Van Tassell B, Kannan H . A mouse model of radiation-induced cardiomyopathy. Int J Cardiol. 2012; 156(2):231-3. PMC: 4899611. DOI: 10.1016/j.ijcard.2012.01.038. View

14.

Zhao B, Jiang W, Zhao Y, Hou J, Xin W . Scavenging effects of salvia miltiorrhiza on free radicals and its protection for myocardial mitochondrial membranes from ischemia-reperfusion injury. Biochem Mol Biol Int. 1996; 38(6):1171-82. View

15.

Liu L, Shi L, Li S, Zhang W, Zhang Y, Zhang Z . Protective Role of Rheum Tanguticum Polysaccharide 1 in Radiation- induced Intestinal Mucosal Injury. Iran J Pharm Res. 2015; 14(3):833-41. PMC: 4518111. View

16.

Cilliers G, Harper I, Lochner A . Radiation-induced changes in the ultrastructure and mechanical function of the rat heart. Radiother Oncol. 1989; 16(4):311-26. DOI: 10.1016/0167-8140(89)90044-3. View

17.

Su X, Yao Z, Li S, Sun H . Synergism of Chinese Herbal Medicine: Illustrated by Danshen Compound. Evid Based Complement Alternat Med. 2016; 2016:7279361. PMC: 4846759. DOI: 10.1155/2016/7279361. View

18.

Marber M, Rose B, Wang Y . The p38 mitogen-activated protein kinase pathway--a potential target for intervention in infarction, hypertrophy, and heart failure. J Mol Cell Cardiol. 2010; 51(4):485-90. PMC: 3061241. DOI: 10.1016/j.yjmcc.2010.10.021. View

19.

Hoffmann A, Engelfriet P, Mulder B . Radiation exposure during follow-up of adults with congenital heart disease. Int J Cardiol. 2006; 118(2):151-3. DOI: 10.1016/j.ijcard.2006.07.012. View

20.

Galper S, Yu J, Mauch P, Strasser J, Silver B, LaCasce A . Clinically significant cardiac disease in patients with Hodgkin lymphoma treated with mediastinal irradiation. Blood. 2010; 117(2):412-8. DOI: 10.1182/blood-2010-06-291328. View