Promoter of the Mycoplasma Pneumoniae RRNA Operon

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1988 Jul 1

PMID 2838465

Citations 5

Authors

H C Hyman

R Gafny

G Glaser

S Razin

Affiliations

Soon will be listed here.

Abstract

RNA transcripts starting from the 5' end of the single Mycoplasma pneumoniae rRNA operon were analyzed by several methods. By primer extension analysis a start site was found 62 nucleotides upstream from the start site of the 16S rRNA. This site was preceded by a putative Pribnow box; however, a defined -35 recognition region was absent. The cloned rRNA operon was transcribed in vitro by using purified RNA polymerase of Escherichia coli. A single start site could be demonstrated within a few nucleotides of the start site found by primer extension analysis of M. pneumoniae transcripts. When fragments from the cloned operon were used as hybridization probes, S1 nuclease mapping yielded a single transcript extending approximately 193 nucleotides upstream from the 16S rRNA start site. The region surrounding this endpoint did not resemble any known promoter sequence. Dot blot hybridization of M. pneumoniae RNA to three oligonucleotides consisting of nucleotides -5 to -21, -38 to -54, and -112 to -132 (from the start of the 16S rRNA gene) indicated that most rRNA transcripts were processed at the stem site preceding the 16S rRNA gene. The majority of the longer precursor transcripts, extending beyond this point, did not extend further upstream to an oligonucleotide consisting of nucleotides -112 to -132. It was concluded that transcription of the rRNA operon of M. pneumoniae is initiated by a single promoter. The nucleotide sequence of the region is presented.

Citing Articles

High Prevalence of Diverse Insertion Sequences within the rRNA Operons of Mycoplasma bovis.

Amram E, Borovok I, Nachum-Biala Y, Ayling R, Lerner U, Harrus S Appl Environ Microbiol. 2016; 82(21):6386-6394.

PMID: 27542937 PMC: 5066355. DOI: 10.1128/AEM.01628-16.

Probing in vivo promoter activities in Mycoplasma pneumoniae: a system for generation of single-copy reporter constructs.

Halbedel S, Stulke J Appl Environ Microbiol. 2006; 72(2):1696-9.

PMID: 16461731 PMC: 1392949. DOI: 10.1128/AEM.72.2.1696-1699.2006.

Transcriptional analysis of the hmw gene cluster of Mycoplasma pneumoniae.

Waldo 3rd R, Popham P, Mothershed E, Lee K, Krause D J Bacteriol. 1999; 181(16):4978-85.

PMID: 10438770 PMC: 93987. DOI: 10.1128/JB.181.16.4978-4985.1999.

Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae.

Himmelreich R, HILBERT H, Plagens H, Pirkl E, Li B, Herrmann R Nucleic Acids Res. 1996; 24(22):4420-49.

PMID: 8948633 PMC: 146264. DOI: 10.1093/nar/24.22.4420.

Cloning, expression, and characterization of the Escherichia coli K-12 rfaD gene.

PEGUES J, Chen L, Gordon A, Ding L, Coleman Jr W J Bacteriol. 1990; 172(8):4652-60.

PMID: 2198271 PMC: 213300. DOI: 10.1128/jb.172.8.4652-4660.1990.

References

Salser W, GESTELAND R, BOLLE A . In vitro synthesis of bacteriophage lysozyme. Nature. 1967; 215(5101):588-91. DOI: 10.1038/215588a0. View

McKenney K, Shimatake H, Court D, Schmeissner U, Brady C, Rosenberg M . A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase. Gene Amplif Anal. 1981; 2:383-415. View

Berk A, Sharp P . Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977; 12(3):721-32. DOI: 10.1016/0092-8674(77)90272-0. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Woese C, Maniloff J, Zablen L . Phylogenetic analysis of the mycoplasmas. Proc Natl Acad Sci U S A. 1980; 77(1):494-8. PMC: 348298. DOI: 10.1073/pnas.77.1.494. View

Glaser G, Razin A, Razin S . Stable RNA synthesis and its control in Mycoplasma capricolum. Nucleic Acids Res. 1981; 9(15):3641-6. PMC: 327380. DOI: 10.1093/nar/9.15.3641. View

Stewart G, Bott K . DNA sequence of the tandem ribosomal RNA promoter for B. subtilis operon rrnB. Nucleic Acids Res. 1983; 11(18):6289-300. PMC: 326373. DOI: 10.1093/nar/11.18.6289. View

Ogasawara N, Moriya S, Yoshikawa H . Structure and organization of rRNA operons in the region of the replication origin of the Bacillus subtilis chromosome. Nucleic Acids Res. 1983; 11(18):6301-18. PMC: 326374. DOI: 10.1093/nar/11.18.6301. View

Woese C, Gutell R, Gupta R, Noller H . Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids. Microbiol Rev. 1983; 47(4):621-69. PMC: 283711. DOI: 10.1128/mr.47.4.621-669.1983. View

10.

Devereux J, Haeberli P, SMITHIES O . A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984; 12(1 Pt 1):387-95. PMC: 321012. DOI: 10.1093/nar/12.1part1.387. View

11.

Amikam D, Glaser G, Razin S . Mycoplasmas (Mollicutes) have a low number of rRNA genes. J Bacteriol. 1984; 158(1):376-8. PMC: 215430. DOI: 10.1128/jb.158.1.376-378.1984. View

12.

Henikoff S . Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984; 28(3):351-9. DOI: 10.1016/0378-1119(84)90153-7. View

13.

Iwami M, Muto A, Yamao F, Osawa S . Nucleotide sequence of the rrnB 16S ribosomal RNA gene from Mycoplasma capricolum. Mol Gen Genet. 1984; 196(2):317-22. DOI: 10.1007/BF00328065. View

14.

Kawauchi Y, Muto A, Yamao F, Osawa S . Molecular cloning of ribosomal protein genes from Mycoplasma capricolum. Mol Gen Genet. 1984; 196(3):521-5. DOI: 10.1007/BF00436202. View

15.

Wise K . Antigen expression from cloned genes of Mycoplasma hyorhinis: an approach to mycoplasma genomic analysis. Isr J Med Sci. 1984; 20(9):754-7. View

16.

Woese C, Stackebrandt E, Ludwig W . What are mycoplasmas: the relationship of tempo and mode in bacterial evolution. J Mol Evol. 1984; 21(4):305-16. DOI: 10.1007/BF02115648. View

17.

Mouches C, Candresse T, Barroso G, Saillard C, Wroblewski H, Bove J . Gene for spiralin, the major membrane protein of the helical mollicute Spiroplasma citri: cloning and expression in Escherichia coli. J Bacteriol. 1985; 164(3):1094-9. PMC: 219302. DOI: 10.1128/jb.164.3.1094-1099.1985. View

18.

Razin S . Molecular biology and genetics of mycoplasmas (Mollicutes). Microbiol Rev. 1985; 49(4):419-55. PMC: 373046. DOI: 10.1128/mr.49.4.419-455.1985. View

19.

Taschke C, Herrmann R . Analysis of transcription and processing signals of the 16S-23S rRNA operon of Mycoplasma hyopneumoniae. Mol Gen Genet. 1986; 205(3):434-41. DOI: 10.1007/BF00338079. View

20.

Taschke C, Ruland K, Herrmann R . Nucleotide sequence of the 16S rRNA of Mycoplasma hyopneumoniae. Nucleic Acids Res. 1987; 15(9):3918. PMC: 340792. DOI: 10.1093/nar/15.9.3918. View