AKAP95 Interacts with Nucleoporin TPR in Mitosis and is Important for the Spindle Assembly Checkpoint

Overview

Journal Cell Cycle

Specialty Cell Biology

Date 2017 Apr 6

PMID 28379780

Citations 4

Authors

Graciela Lopez-Soop

Torunn Ronningen

Agnieszka Rogala

Nina Richartz

Heidi Kiil Blomhoff

Bernd Thiede

Philippe Collas

Thomas Kuntziger

Affiliations

Soon will be listed here.

Abstract

Faithful chromosome segregation during mitosis relies on a proofreading mechanism that monitors proper kinetochore-microtubule attachments. The spindle assembly checkpoint (SAC) is based on the concerted action of numerous components that maintain a repressive signal inhibiting transition into anaphase until all chromosomes are attached. Here we show that A-Kinase Anchoring Protein 95 (AKAP95) is necessary for proper SAC function. AKAP95-depleted HeLa cells show micronuclei formed from lagging chromosomes at mitosis. Using a BioID proximity-based proteomic screen, we identify the nuclear pore complex protein TPR as a novel AKAP95 binding partner. We show interaction between AKAP95 and TPR in mitosis, and an AKAP95-dependent enrichment of TPR in the spindle microtubule area in metaphase, then later in the spindle midzone area. AKAP95-depleted cells display faster prometaphase to anaphase transition, escape from nocodazole-induced mitotic arrest and show a partial delocalization from kinetochores of the SAC component MAD1. Our results demonstrate an involvement of AKAP95 in proper SAC function likely through its interaction with TPR.

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