Effect of Vitamin D-supplementation on Bone Markers (serum P1NP and CTX): A Randomized, Double Blinded, Placebo Controlled Trial Among Healthy Immigrants Living in Norway

Overview

Journal Bone Rep

Date 2017 Apr 6

PMID 28377958

Citations 10

Authors

Ahmed A Madar

Kirsten V Knutsen

Lars C Stene

Mette Brekke

Per Lagerlov

Haakon E Meyer

Helen M Macdonald

Affiliations

Soon will be listed here.

Abstract

Objective: Vitamin D is essential for the maintenance of calcium homeostasis and bone mineralization; and low serum 25-hydroxyvitamin D (s-25-(OH)D) concentrations are associated with increased bone turnover. However, there is a lack of randomized controlled trials that have investigated the effect of vitamin D treatment on bone turnover in immigrant populations. We aimed to investigate the effect of 16-week daily vitamin D supplementation on bone formation marker serum procollagen type 1 amino-terminal propeptide (P1NP) and bone resorption marker C-terminal crosslinked telopeptide of type I collagen (CTX).

Design: Double-blind, randomized, placebo-controlled trial.

Setting: Immigrant community centers in Oslo, Norway.

Participants: 251 healthy adults aged 18-50 years with a non-Western immigrant background were recruited.

Intervention: 16 weeks of daily oral supplementation with either 10 μg vitamin D, 25 μg vitamin D, or placebo.

Main Outcome Measures: Difference in change during the 16-week intervention between the intervention groups combined (10 or 25 μg of vitamin D/day) and placebo, in serum P1NP and serum CTX.

Results: A total of 214 (85%) participants completed the study. S-25-(OH)D increased from 29 nmol/L at baseline to 49 nmol/L in the intervention group with no significant change in the placebo group. However, there was no difference in change of serum P1NP (mean difference: - 1.2 μg/L (95% CI: - 5.4, 2.9, P = 0.6)) and serum CTX (mean difference: - 0.005 μg/L (95% CI: - 0.03, 0.02, P = 0.7)) between those receiving vitamin D supplementation compared with placebo. The plasma PTH had decreased by a mean of - 1.97 pmol/L (95% CI: - 2.7, - 1.3, P < 0.0001) in the vitamin D group compared to placebo.

Conclusions: Supplementation with 10 or 25 μg oral vitamin D during winter and spring for 16 weeks did not significantly affect serum P1NP and serum CTX, despite increasing s-25(OH)D and decreasing PTH in a healthy immigrant population with low baseline vitamin D status. Trial registration number: NCT01263288.

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References

Macdonald H, Wood A, Aucott L, Black A, Fraser W, Mavroeidi A . Hip bone loss is attenuated with 1000 IU but not 400 IU daily vitamin D3: a 1-year double-blind RCT in postmenopausal women. J Bone Miner Res. 2013; 28(10):2202-13. DOI: 10.1002/jbmr.1959. View

Herrmann M, Seibel M, Seibel M . The amino- and carboxyterminal cross-linked telopeptides of collagen type I, NTX-I and CTX-I: a comparative review. Clin Chim Acta. 2008; 393(2):57-75. DOI: 10.1016/j.cca.2008.03.020. View

Barnes M, Robson P, Bonham M, Strain J, Wallace J . Effect of vitamin D supplementation on vitamin D status and bone turnover markers in young adults. Eur J Clin Nutr. 2006; 60(6):727-33. DOI: 10.1038/sj.ejcn.1602374. View

Cosman F, Morgan D, Nieves J, Shen V, Luckey M, Dempster D . Resistance to bone resorbing effects of PTH in black women. J Bone Miner Res. 1997; 12(6):958-66. DOI: 10.1359/jbmr.1997.12.6.958. View

Lips P . Vitamin D deficiency and secondary hyperparathyroidism in the elderly: consequences for bone loss and fractures and therapeutic implications. Endocr Rev. 2001; 22(4):477-501. DOI: 10.1210/edrv.22.4.0437. View

Erkal M, Wilde J, Bilgin Y, Akinci A, Demir E, Bodeker R . High prevalence of vitamin D deficiency, secondary hyperparathyroidism and generalized bone pain in Turkish immigrants in Germany: identification of risk factors. Osteoporos Int. 2006; 17(8):1133-40. DOI: 10.1007/s00198-006-0069-2. View

Holvik K, Meyer H, Haug E, Brunvand L . Prevalence and predictors of vitamin D deficiency in five immigrant groups living in Oslo, Norway: the Oslo Immigrant Health Study. Eur J Clin Nutr. 2004; 59(1):57-63. DOI: 10.1038/sj.ejcn.1602033. View

Slemenda C, Peacock M, Hui S, Zhou L, JOHNSTON C . Reduced rates of skeletal remodeling are associated with increased bone mineral density during the development of peak skeletal mass. J Bone Miner Res. 1997; 12(4):676-82. DOI: 10.1359/jbmr.1997.12.4.676. View

Trautvetter U, Neef N, Leiterer M, Kiehntopf M, Kratzsch J, Jahreis G . Effect of calcium phosphate and vitamin D₃ supplementation on bone remodelling and metabolism of calcium, phosphorus, magnesium and iron. Nutr J. 2014; 13:6. PMC: 3898568. DOI: 10.1186/1475-2891-13-6. View

10.

Jenkins N, Black M, Paul E, Pasco J, Kotowicz M, Schneider H . Age-related reference intervals for bone turnover markers from an Australian reference population. Bone. 2013; 55(2):271-6. DOI: 10.1016/j.bone.2013.04.003. View

11.

Holick M, Chen T . Vitamin D deficiency: a worldwide problem with health consequences. Am J Clin Nutr. 2008; 87(4):1080S-6S. DOI: 10.1093/ajcn/87.4.1080S. View

12.

Riggs B, OFallon W, Muhs J, OConnor M, Kumar R, Melton 3rd L . Long-term effects of calcium supplementation on serum parathyroid hormone level, bone turnover, and bone loss in elderly women. J Bone Miner Res. 1998; 13(2):168-74. DOI: 10.1359/jbmr.1998.13.2.168. View

13.

Eggemoen A, Knutsen K, Dalen I, Jenum A . Vitamin D status in recently arrived immigrants from Africa and Asia: a cross-sectional study from Norway of children, adolescents and adults. BMJ Open. 2013; 3(10):e003293. PMC: 3808781. DOI: 10.1136/bmjopen-2013-003293. View

14.

Macdonald H, Mavroeidi A, Barr R, Black A, Fraser W, Reid D . Vitamin D status in postmenopausal women living at higher latitudes in the UK in relation to bone health, overweight, sunlight exposure and dietary vitamin D. Bone. 2008; 42(5):996-1003. DOI: 10.1016/j.bone.2008.01.011. View

15.

Stokes F, Ivanov P, Bailey L, Fraser W . The effects of sampling procedures and storage conditions on short-term stability of blood-based biochemical markers of bone metabolism. Clin Chem. 2010; 57(1):138-40. DOI: 10.1373/clinchem.2010.157289. View

16.

Seamans K, Hill T, Wallace J, Horigan G, Lucey A, Barnes M . Cholecalciferol supplementation throughout winter does not affect markers of bone turnover in healthy young and elderly adults. J Nutr. 2010; 140(3):454-60. DOI: 10.3945/jn.109.113480. View

17.

Lowe N, Mitra S, Foster P, Bhojani I, McCann J . Vitamin D status and markers of bone turnover in Caucasian and South Asian postmenopausal women living in the UK. Br J Nutr. 2010; 103(12):1706-10. DOI: 10.1017/S0007114509993850. View

18.

Brot C, Vestergaard P, Kolthoff N, Gram J, Hermann A, Sorensen O . Vitamin D status and its adequacy in healthy Danish perimenopausal women: relationships to dietary intake, sun exposure and serum parathyroid hormone. Br J Nutr. 2001; 86 Suppl 1:S97-103. DOI: 10.1079/bjn2001345. View

19.

Holvik K, Meyer H, Sogaard A, Selmer R, Haug E, Falch J . Biochemical markers of bone turnover and their relation to forearm bone mineral density in persons of Pakistani and Norwegian background living in Oslo, Norway: The Oslo Health Study. Eur J Endocrinol. 2006; 155(5):693-9. DOI: 10.1530/eje.1.02282. View

20.

Islam M, Viljakainen H, Karkkainen M, Saarnio E, Laitinen K, Lamberg-Allardt C . Prevalence of vitamin D deficiency and secondary hyperparathyroidism during winter in pre-menopausal Bangladeshi and Somali immigrant and ethnic Finnish women: associations with forearm bone mineral density. Br J Nutr. 2011; 107(2):277-83. DOI: 10.1017/S0007114511002893. View