New and Old Genes Associated with Topotecan Resistance Development in Ovarian Cancer Cell Lines

Overview

Journal Anticancer Res

Specialty Oncology

Date 2017 Apr 5

PMID 28373423

Citations 14

Authors

Andrzej Klejewski

Monika Swierczewska

Katarzyna Zaorska

Maciej Brazert

Michal Nowicki

Maciej Zabel

Radoslaw Januchowski

Affiliations

Soon will be listed here.

Abstract

Background: Low effectiveness of chemotherapy in ovarian cancer results from development of drug resistance. Topotecan is a drug used as second-line chemotherapy for this cancer type. We analyzed development of topotecan resistance in ovarian cancer cell lines.

Materials And Methods: A chemosensitivity assay, MTT test, was performed to assess drug resistance. Quantitative polymerase chain reaction (Q-PCR) assays were performed to determine ABCB1, ABCG2, ALDH1A1, IFIH1, SAMD4 and EPHA3 gene expression.

Results: We observed dose-dependent responses to topotecan. In all topotecan-resistant cell lines an overexpression of ABCG2, IFIH1 and SAMD4 genes was observed. Expression of ABCB1 gene was observed in one cell line. Expression of ALDH1A1 was up-regulated in A2780 and down-regulated in SKOV-3-resistant cell lines. Short-time exposure led to similar patterns of gene expression for the investigated genes.

Conclusion: Expression of ABCG2 and ABCB1 genes plays the most important role in topotecan resistance. The role of other investigated genes seems to be complementary.

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