NETWORKED 3B: a Novel Protein in the Actin Cytoskeleton-endoplasmic Reticulum Interaction

Overview

Journal J Exp Bot

Publisher Oxford University Press

Specialty Biology

Date 2017 Apr 4

PMID 28369569

Citations 12

Authors

Pengwei Wang

Patrick J Hussey

Affiliations

Soon will be listed here.

Abstract

In plants movement of the endoplasmic reticulum (ER) is dependent on the actin cytoskeleton. However little is known about proteins that link the ER membrane and the actin cytoskeleton. Here we identified a novel protein, NETWORKED 3B (NET3B), which is associated with the ER and actin cytoskeleton in vivo. NET3B belongs to a superfamily of plant specific actin binding proteins, the NETWORKED family. NET3B associates with the actin cytoskeleton in vivo through an N-terminal NET actin binding (NAB) domain, which has been well-characterized in other members of the NET family. A three amino acid insertion, Val-Glu-Asp, in the NAB domain of NET3B appears to lower its ability to localize to the actin cytoskeleton compared with NET1A, the founding member of the NET family. The C-terminal domain of NET3B links the protein to the ER. Overexpression of NET3B enhanced the association between the ER and the actin cytoskeleton, and the extent of this association was dependent on the amount of NET3B available. Another effect of NET3B overexpression was a reduction in ER membrane diffusion. In conclusion, our results revealed that NET3B modulates ER and actin cytoskeleton interactions in higher plants.

Citing Articles

Regulation of cytoskeleton dynamics and its interplay with force in plant cells.

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Organelle Interactions in Plant Cells.

Hall M, Kunjumon T, Ghosh P, Currie L, Mathur J Results Probl Cell Differ. 2024; 73:43-69.

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NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity.

Hawkins T, Kopischke M, Duckney P, Rybak K, Mentlak D, Kroon J Nat Commun. 2023; 14(1):5848.

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Fine mapping and candidate gene analysis of gynoecy trait in chieh-qua ( Cogn. var. How).

Wang M, Yang S, Liu W, Cao Z, Chen L, Liu W Front Plant Sci. 2023; 14:1158735.

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Membrane contact sites and cytoskeleton-membrane interactions in autophagy.

Duckney P, Wang P, Hussey P FEBS Lett. 2022; 596(17):2093-2103.

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