Atomic Resolution Structure of Serine Protease Proteinase K at Ambient Temperature

Overview

Journal Sci Rep

Specialty Science

Date 2017 Apr 1

PMID 28361898

Citations 16

Authors

Tetsuya Masuda

Mamoru Suzuki

Shigeyuki Inoue

Changyong Song

Takanori Nakane

Eriko Nango

Rie Tanaka

Kensuke Tono

Yasumasa Joti

Takashi Kameshima

Takaki Hatsui

Makina Yabashi

Bunzo Mikami

Osamu Nureki

Keiji Numata

So Iwata

Michihiro Sugahara

Affiliations

Soon will be listed here.

Abstract

Atomic resolution structures (beyond 1.20 Å) at ambient temperature, which is usually hampered by the radiation damage in synchrotron X-ray crystallography (SRX), will add to our understanding of the structure-function relationships of enzymes. Serial femtosecond crystallography (SFX) has attracted surging interest by providing a route to bypass such challenges. Yet the progress on atomic resolution analysis with SFX has been rather slow. In this report, we describe the 1.20 Å resolution structure of proteinase K using 13 keV photon energy. Hydrogen atoms, water molecules, and a number of alternative side-chain conformations have been resolved. The increase in the value of B-factor in SFX suggests that the residues and water molecules adjacent to active sites were flexible and exhibited dynamic motions at specific substrate-recognition sites.

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