Saponins from Improve Hematopoiesis by Promoting Survival Through FAK and Erk1/2 Activation and Modulating Cytokine Production in Bone Marrow

Overview

Journal Front Pharmacol

Date 2017 Apr 1

PMID 28360858

Citations 13

Authors

Xin Chen

Bogang Li

Yue Gao

Jianxin Ji

Zhongliu Wu

Shuang Chen

Affiliations

Soon will be listed here.

Abstract

Radix Sanguisorbae, the root of L. is used as traditional Chinese medicine. In recent decades, it has been reported to be clinically effective against myelosuppression induced by chemotherapy and/ or radiotherapy. However, the underlining mechanism has not been well studied. In this work, we evaluated the hematopoietic effect of total saponins from L. on myelosuppressive mice induced by cyclophosphamide and byCo-γ-irradiation and confirmed the therapeutic effect. Then, we found total saponins and their characteristic constituents Ziyuglycoside I and Ziyuglycoside II can inhibit apoptosis of TF-1 cells caused by cytokine deprivation, and promote survival of mouse bone marrow nuclear cells through focal adhesion kinase (FAK) and extracellular signal-regulated kinase 1/2 (Erk1/2) activation . In addition, they can down-regulate macrophage inflammatory protein 2 (MIP-2), platelet factor 4 (PF4) and P-selectin secretion, which are reported to be suppressive to hematopoiesis, both and . These results suggest that promotion of survival through FAK and Erk1/2 activation and inhibition of suppressive cytokines in the bone marrow is likely to be the pharmacological mechanism underlying the hematopoietic effect of saponins from L.

Citing Articles

Exploration of the Active Component and Mechanisms of Shengyu Decoction Against Myelosuppression Using Network Pharmacology and in vitro Experimental Validation.

Ma B, Hou P, Liu R, Cang A, Zhao L Drug Des Devel Ther. 2024; 18:2405-2420.

PMID: 38915868 PMC: 11195678. DOI: 10.2147/DDDT.S458953.

L. enhances the 5-fluorouracil sensitivity and overcomes chemoresistance in 5-fluorouracil-resistant colorectal cancer cells via Ras/MEK/ERK and PI3K/Akt pathways.

Zhang W, Ou L, Peng C, Sang S, Feng Z, Zou Y Heliyon. 2023; 9(6):e16798.

PMID: 37484409 PMC: 10360953. DOI: 10.1016/j.heliyon.2023.e16798.

Rapid Profiling of Metabolites Combined with Network Pharmacology to Explore the Potential Mechanism of L. against Thrombocytopenia.

Dai Y, Zhang K, Wang L, Xiong L, Huang F, Huang Q Metabolites. 2022; 12(11).

PMID: 36355157 PMC: 9693491. DOI: 10.3390/metabo12111074.

Aqueous extract of blocks the Wnt/β-catenin signaling pathway in colorectal cancer cells.

Liu M, Li W, Dai C, Kei Lam C, Li Z, Chen J RSC Adv. 2022; 8(19):10197-10206.

PMID: 35540488 PMC: 9078833. DOI: 10.1039/c8ra00438b.

Investigation of Tannins Transformation in Over Carbonizing by Stir-Frying.

Gu W, Wang H, Su M, Wang Y, Xu F, Hu Q Front Mol Biosci. 2022; 9:762224.

PMID: 35309515 PMC: 8924296. DOI: 10.3389/fmolb.2022.762224.

References

Civini S, Jin P, Ren J, Sabatino M, Castiello L, Jin J . Leukemia cells induce changes in human bone marrow stromal cells. J Transl Med. 2013; 11:298. PMC: 3882878. DOI: 10.1186/1479-5876-11-298. View

Wang Y, Probin V, Zhou D . Cancer therapy-induced residual bone marrow injury-Mechanisms of induction and implication for therapy. Curr Cancer Ther Rev. 2009; 2(3):271-279. PMC: 2779029. DOI: 10.2174/157339406777934717. View

Kim Y, Chung C, Kim J, Ko K, Park S, Kim J . Anti-wrinkle activity of ziyuglycoside I isolated from a Sanguisorba officinalis root extract and its application as a cosmeceutical ingredient. Biosci Biotechnol Biochem. 2008; 72(2):303-11. DOI: 10.1271/bbb.70268. View

Liu Y, Nielsen M, Staerk D, Jager A . High-resolution bacterial growth inhibition profiling combined with HPLC-HRMS-SPE-NMR for identification of antibacterial constituents in Chinese plants used to treat snakebites. J Ethnopharmacol. 2014; 155(2):1276-83. DOI: 10.1016/j.jep.2014.07.019. View

Broxmeyer H, Sherry B, Cooper S, Lu L, Maze R, Beckmann M . Comparative analysis of the human macrophage inflammatory protein family of cytokines (chemokines) on proliferation of human myeloid progenitor cells. Interacting effects involving suppression, synergistic suppression, and blocking of suppression. J Immunol. 1993; 150(8 Pt 1):3448-58. View

Khoury H, Adkins D, Brown R, Vij R, Westervelt P, Trinkaus K . Adverse side-effects associated with G-CSF in patients with chronic myeloid leukemia undergoing allogeneic peripheral blood stem cell transplantation. Bone Marrow Transplant. 2000; 25(11):1197-201. DOI: 10.1038/sj.bmt.1702423. View

Petersen F, Ludwig A, Flad H, Brandt E . TNF-alpha renders human neutrophils responsive to platelet factor 4. Comparison of PF-4 and IL-8 reveals different activity profiles of the two chemokines. J Immunol. 1996; 156(5):1954-62. View

Ban J, Nguyen H, Lee H, Cho S, Ju H, Kim J . Neuroprotective properties of gallic acid from Sanguisorbae radix on amyloid beta protein (25--35)-induced toxicity in cultured rat cortical neurons. Biol Pharm Bull. 2008; 31(1):149-53. DOI: 10.1248/bpb.31.149. View

Levesque J, Zannettino A, Pudney M, Niutta S, Haylock D, Snapp K . PSGL-1-mediated adhesion of human hematopoietic progenitors to P-selectin results in suppression of hematopoiesis. Immunity. 1999; 11(3):369-78. DOI: 10.1016/s1074-7613(00)80112-0. View

10.

Zhu X, Wang K, Zhang K, Zhang T, Yin Y, Xu F . Ziyuglycoside I Inhibits the Proliferation of MDA-MB-231 Breast Carcinoma Cells through Inducing p53-Mediated G2/M Cell Cycle Arrest and Intrinsic/Extrinsic Apoptosis. Int J Mol Sci. 2016; 17(11). PMC: 5133901. DOI: 10.3390/ijms17111903. View

11.

Geest C, Coffer P . MAPK signaling pathways in the regulation of hematopoiesis. J Leukoc Biol. 2009; 86(2):237-50. DOI: 10.1189/jlb.0209097. View

12.

Majka M, Janowska-Wieczorek A, Ratajczak J, Ehrenman K, Pietrzkowski Z, Kowalska M . Numerous growth factors, cytokines, and chemokines are secreted by human CD34(+) cells, myeloblasts, erythroblasts, and megakaryoblasts and regulate normal hematopoiesis in an autocrine/paracrine manner. Blood. 2001; 97(10):3075-85. DOI: 10.1182/blood.v97.10.3075. View

13.

. American Society of Clinical Oncology. Recommendations for the use of hematopoietic colony-stimulating factors: evidence-based, clinical practice guidelines. J Clin Oncol. 1994; 12(11):2471-508. DOI: 10.1200/JCO.1994.12.11.2471. View

14.

Deuel T, Senior R, Chang D, Griffin G, Heinrikson R, Kaiser E . Platelet factor 4 is chemotactic for neutrophils and monocytes. Proc Natl Acad Sci U S A. 1981; 78(7):4584-7. PMC: 319837. DOI: 10.1073/pnas.78.7.4584. View

15.

Eto T, Winkler I, Purton L, Levesque J . Contrasting effects of P-selectin and E-selectin on the differentiation of murine hematopoietic progenitor cells. Exp Hematol. 2005; 33(2):232-42. DOI: 10.1016/j.exphem.2004.10.018. View

16.

Broxmeyer H, Cooper S, Hague N, Benninger L, Sarris A, Cornetta K . Human chemokines: enhancement of specific activity and effects in vitro on normal and leukemic progenitors and a factor-dependent cell line and in vivo in mice. Ann Hematol. 1995; 71(5):235-46. DOI: 10.1007/BF01744373. View

17.

Vemula S, Ramdas B, Hanneman P, Martin J, Beggs H, Kapur R . Essential role for focal adhesion kinase in regulating stress hematopoiesis. Blood. 2010; 116(20):4103-15. PMC: 2993618. DOI: 10.1182/blood-2010-01-262790. View

18.

East J . The effect of certain plant preparations on the fertility of laboratory mammals. 4. Sanguisorba officinalis L. J Endocrinol. 1955; 12(4):273-6. DOI: 10.1677/joe.0.0120273. View

19.

Park K, Koh D, Kim K, Park J, Lim Y . Antiallergic activity of a disaccharide isolated from Sanguisorba officinalis. Phytother Res. 2004; 18(8):658-62. DOI: 10.1002/ptr.1545. View

20.

Zhu X, Wang K, Zhang K, Zhu L, Zhou F . Ziyuglycoside II induces cell cycle arrest and apoptosis through activation of ROS/JNK pathway in human breast cancer cells. Toxicol Lett. 2014; 227(1):65-73. DOI: 10.1016/j.toxlet.2014.03.015. View