» Articles » PMID: 28360403

A Universal, Rapid, and Inexpensive Method for Genomic DNA Isolation from the Whole Blood of Mammals and Birds

Overview
Journal J Genet
Specialty Genetics
Date 2017 Apr 1
PMID 28360403
Citations 10
Authors
Affiliations
Soon will be listed here.
Abstract

There is no 'one' procedure for extracting DNA from the whole blood of both mammals and birds, since each species has a unique property that require different methods to release its own DNA. Therefore, to obtain genomic DNA, a universal, rapid, and noncostly method was developed. A very simple biological basis is followed in this procedure, in which, when the blood is placed in water, it rapidly enters the RBCs by osmosis and causes cells to burst by hemolysis. The validity of extracting genomic DNA was confirmed by several molecular biological experiments. It was found that this method provides an efficient and versatile alternative for extracting bulk amounts of highly-qualified DNA from the blood of a wide range of species. This is the first manuscript that describes use of distilled water as the only eliminator of RBCs among all other known DNA extraction techniques.

Citing Articles

Reproduction of Awassi and Hamdani Sheep Is Associated With a Novel Missense SNP (p.24Ile>Thr) of the Gene.

Al-Jabar W, Al-Thuwaini T Bioinform Biol Insights. 2024; 18:11779322241267188.

PMID: 39114480 PMC: 11304492. DOI: 10.1177/11779322241267188.


The Novel Variant g.22645332G>T Is Strongly Related to Awassi and Hamdani Sheep Litter Size.

Imran F, Al-Thuwaini T Bioinform Biol Insights. 2024; 18:11779322241248912.

PMID: 38681096 PMC: 11047254. DOI: 10.1177/11779322241248912.


Association of Novel C319T Variant of Gene 3'UTR Region With Reproductive Performance in Awassi Sheep.

Alkhammas A, Al-Thuwaini T, Al-Shuhaib M, Khazaal N Bioinform Biol Insights. 2023; 17:11779322231179018.

PMID: 37313032 PMC: 10259137. DOI: 10.1177/11779322231179018.


A Missense p.Q>R234 Mutation in the Osteopontin Gene Is Associated With the Prolificacy of Iraqi Awassi Ewes.

Al-Thuwaini T, Al-Shuhaib M, Kadhem A, Alkhammas A Bioinform Biol Insights. 2023; 17:11779322231172848.

PMID: 37197213 PMC: 10184200. DOI: 10.1177/11779322231172848.


A Novel c.100C > G Mutation in the Gene and Its Relation With the Reproductive Traits of Awassi Ewes.

Al-Thuwaini T, Albazi W, Al-Shuhaib M, Merzah L, Mohammed R, Rhadi F Bioinform Biol Insights. 2023; 17:11779322231170988.

PMID: 37153841 PMC: 10159244. DOI: 10.1177/11779322231170988.


References
1.
Orita M, Iwahana H, Kanazawa H, Hayashi K, Sekiya T . Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci U S A. 1989; 86(8):2766-70. PMC: 286999. DOI: 10.1073/pnas.86.8.2766. View

2.
Miller S, Dykes D, Polesky H . A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 1988; 16(3):1215. PMC: 334765. DOI: 10.1093/nar/16.3.1215. View

3.
Psifidi A, Dovas C, Bramis G, Lazou T, Russel C, Arsenos G . Comparison of eleven methods for genomic DNA extraction suitable for large-scale whole-genome genotyping and long-term DNA banking using blood samples. PLoS One. 2015; 10(1):e0115960. PMC: 4312062. DOI: 10.1371/journal.pone.0115960. View

4.
Tan S, Yiap B . DNA, RNA, and protein extraction: the past and the present. J Biomed Biotechnol. 2009; 2009:574398. PMC: 2789530. DOI: 10.1155/2009/574398. View

5.
Grimberg J, Nawoschik S, Belluscio L, McKee R, Turck A, Eisenberg A . A simple and efficient non-organic procedure for the isolation of genomic DNA from blood. Nucleic Acids Res. 1989; 17(20):8390. PMC: 334995. DOI: 10.1093/nar/17.20.8390. View