Different Reading Frames Are Responsible for IS1-dependent Deletions and Recombination

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1988 Apr 1

PMID 2835288

Citations 3

Authors

G Braedt

Affiliations

Soon will be listed here.

Abstract

Two classes of plasmids in addition to the parent become apparent when plasmids that contain direct repeats of IS1. One class of plasmids has deleted sequences from the end of IS1 to nonrandom sites within the plasmid. The appearance of these plasmids in the population requires intact insA and insB reading frames, but not insC. The other class of plasmids has undergone an exchange within the direct repeats of IS1 on the plasmid. Their appearance requires InsC but neither InsA nor InsB. The two reactions may represent two distinguishable steps in IS1 transposition. The InsC-catalyzed exchange is independent of RecA and resembles hologous recombination since the frequency of recombinants arising from exchanges in different regions of IS1 appears to be roughly proportional to the size of the region. InsC can also catalyze an exchange between direct repeats of non-IS1 DNA.

Citing Articles

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The 987P gene cluster in enterotoxigenic Escherichia coli contains an STpa transposon that activates 987P expression.

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