KP4 to Control in Wheat: Enhanced Greenhouse Resistance to Loose Smut and Changes in Transcript Abundance of Pathogen Related Genes in Infected KP4 Plants

Overview

Journal Biotechnol Rep (Amst)

Specialty Biotechnology

Date 2017 Mar 30

PMID 28352545

Citations 6

Authors

Carolina Diaz Quijano

Fabienne Wichmann

Thomas Schlaich

Alessandro Fammartino

Jana Huckauf

Kerstin Schmidt

Christoph Unger

Inge Broer

Christof Sautter

Affiliations

Soon will be listed here.

Abstract

causes loose smut, which is a seed-borne fungal disease of wheat, and responsible for yield losses up to 40%. Loose smut is a threat to seed production in developing countries where small scale farmers use their own harvest as seed material. The killer protein 4 (KP4) is a virally encoded toxin from and inhibits growth of susceptible races of fungi from the Ustilaginales. Enhanced resistance in KP4 wheat to stinking smut, which is caused by had been reported earlier. We show that KP4 in genetically engineered wheat increased resistance to loose smut up to 60% compared to the non-KP4 control under greenhouse conditions. This enhanced resistance is dose and race dependent. The overexpression of the transgene and its effect on fungal growth have indirect effects on the expression of endogenous pathogen defense genes.

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