Comparison of the Uptake and Toxicity of Collagen- and Synthetic Polymer-Coated Gold Nanoparticles

Overview

Journal Nanomaterials (Basel)

Date 2017 Mar 29

PMID 28347072

Citations 11

Authors

Oana T Marisca

Karsten Kantner

Christian Pfeiffer

Qian Zhang

Beatriz Pelaz

Nicolae Leopold

Wolfgang J Parak

Joanna Rejman

Affiliations

Soon will be listed here.

Abstract

We studied the physico-chemical properties (size, shape, zeta-potential), cellular internalization and toxicity of gold nanoparticles (NPs) stabilized with the most abundant mammalian protein, collagen. The properties of these gold NPs were compared to the same sized gold NPs coated with synthetic poly(isobutylene-alt-maleic anhydride) (PMA). Intracellular uptake and cytotoxicity were assessed in two cell lines (cervical carcinoma and lung adenocarcinoma cells) by employing inductively-coupled plasma-mass spectrometry (ICP-MS) analysis and a cell viability assay based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), respectively. We found that the collagen-coated gold NPs exhibit lower cytotoxicity, but higher uptake levels than PMA-coated gold NPs. These results demonstrate that the surface coating of Au NPs plays a decisive role in their biocompatibility.

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