Identification and Characterization of the Products of Six Region III Flagellar Genes (flaAII.3 Through FlaQII) of Salmonella Typhimurium

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1988 May 1

PMID 2834334

Citations 19

Authors

M Homma

T Iino

R M Macnab

Affiliations

Soon will be listed here.

Abstract

A portion of flagellar region III of the Salmonella typhimurium genome has been cloned and shown to contain six genes: flaAII.3, flaAIII, flaS, flaR, flaQI, and flaQII. Of these, all but flaQI were known to exist from mutant studies; the former flaQ has been renamed flaQII. The genes were shown by minicell analysis to encode proteins with apparent molecular masses of 28, 48, 15, 46, 17, and 37 kilodaltons, respectively. The presence of a flagellar-gene-specific promoter in the vicinity of flaQI was established by testing expression of the plasmid-encoded tetracycline resistance gene in artificial constructions. In minicell preparations, the flaAII.3 and flaR products were found principally in the cytoplasmic fraction; the rest were found principally in the membrane fraction. A comparison between the homologous genes of S. typhimurium and Escherichia coli confirmed that their genomic organizations were similar and that their products had similar molecular masses and isoelectric points.

Citing Articles

The FliK protein and flagellar hook-length control.

Waters R, OToole P, Ryan K Protein Sci. 2007; 16(5):769-80.

PMID: 17456739 PMC: 2206646. DOI: 10.1110/ps.072785407.

Deletion analysis of the flagellar switch protein FliG of Salmonella.

Kihara M, Miller G, Macnab R J Bacteriol. 2000; 182(11):3022-8.

PMID: 10809678 PMC: 94485. DOI: 10.1128/JB.182.11.3022-3028.2000.

Structural and genetic analysis of a mutant of Rhodobacter sphaeroides WS8 deficient in hook length control.

Gonzalez-Pedrajo B, Ballado T, Campos A, Sockett R, Camarena L, Dreyfus G J Bacteriol. 1997; 179(21):6581-8.

PMID: 9352903 PMC: 179582. DOI: 10.1128/jb.179.21.6581-6588.1997.

The FliO, FliP, FliQ, and FliR proteins of Salmonella typhimurium: putative components for flagellar assembly.

Ohnishi K, Fan F, Schoenhals G, Kihara M, Macnab R J Bacteriol. 1997; 179(19):6092-9.

PMID: 9324257 PMC: 179513. DOI: 10.1128/jb.179.19.6092-6099.1997.

Deletion analysis of the FliM flagellar switch protein of Salmonella typhimurium.

Toker A, Kihara M, Macnab R J Bacteriol. 1996; 178(24):7069-79.

PMID: 8955386 PMC: 178617. DOI: 10.1128/jb.178.24.7069-7079.1996.

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