MiR-146b Protects Cardiomyocytes Injury in Myocardial Ischemia/reperfusion by Targeting Smad4

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2017 Mar 25

PMID 28337293

Citations 20

Authors

Yun-Feng Di

De-Cai Li

Yan-Qing Shen

Chun-Lei Wang

Da-Yong Zhang

An-Quan Shang

Teng Hu

Affiliations

Soon will be listed here.

Abstract

MicroRNAs, a class of small and non-encoding RNAs that transcriptionally or post-transcriptionally modulate the expression of their target genes, have been implicated as critical regulatory molecules in many cardiovascular diseases, including ischemia-/reperfusion-induced cardiac injury. In the present study, we report on the role of miR-146b in myocardial I/R injury and the underlying cardio-protective mechanism. Antagomir-146b was used to explore the effects of miR-146b on cardiac ischemia/reperfusion injury (30 min ischemia followed by 180 min reperfusion). As predicted, miR-146b overexpression significantly reduced the infarct size and cardiomyocytes apoptosis and release of creatine kinase and lactate dehydrogenase. In addition, miR-146b attenuated H9c2 cell apoptosis. Furthermore, Smad4 was predicted and verified as a potential miR-146b target using bioinformatics and luciferase assay. In summary, this study demonstrated that miR-146b plays a critical protective role in cardiac ischemic injury and may provide a new therapeutic approach for the treatment of myocardial I/R injury.

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