Biosynthesis of Silver Nanoparticles Using Taxus Yunnanensis Callus and Their Antibacterial Activity and Cytotoxicity in Human Cancer Cells

Overview

Journal Nanomaterials (Basel)

Date 2017 Mar 25

PMID 28335288

Citations 23

Authors

Qian Hua Xia

Yan Jun Ma

Jian Wen Wang

Affiliations

Soon will be listed here.

Abstract

Plant constituents could act as chelating/reducing or capping agents for synthesis of silver nanoparticles (AgNPs). The green synthesis of AgNPs has been considered as an environmental friendly and cost-effective alternative to other fabrication methods. The present work described the biosynthesis of AgNPs using callus extracts from and evaluated their antibacterial activities in vitro and potential cytotoxicity in cancer cells. Callus extracts were able to reduce silver nitrate at 1 mM in 10 min. Transmission electron microscope (TEM) indicated the synthesized AgNPs were spherical with the size range from 6.4 to 27.2 nm. X-ray diffraction (XRD) confirmed the AgNPs were in the form of nanocrystals. Fourier transform infrared spectroscopy (FTIR) suggested phytochemicals in callus extracts were possible reducing and capping agents. The AgNPs exhibited effective inhibitory activity against all tested human pathogen bacteria and the inhibition against Gram-positive bacteria was stronger than that of Gram-negative bacteria. Furthermore, they exhibited stronger cytotoxic activity against human hepatoma SMMC-7721 cells and induced noticeable apoptosis in SMMC-7721 cells, but showed lower cytotoxic against normal human liver cells (HL-7702). Our results suggested that biosynthesized AgNPs could be an alternative measure in the field of antibacterial and anticancer therapeutics.

Citing Articles

silver chloride nanoparticles and mediated silver/silver chloride nanoparticles inhibit human hepatocellular and lung cancer cell lines.

Kabir S, Alam M, Uddin M Biochem Biophys Rep. 2024; 40:101818.

PMID: 39290346 PMC: 11406076. DOI: 10.1016/j.bbrep.2024.101818.

Synthesis and Characterization of Paclitaxel-Loaded Silver Nanoparticles: Evaluation of Cytotoxic Effects and Antimicrobial Activity.

Tunc T, Hepokur C, Kari Per A Bioinorg Chem Appl. 2024; 2024:9916187.

PMID: 38380152 PMC: 10878759. DOI: 10.1155/2024/9916187.

A comprehensive review of Shengdeng in Tibetan medicine: textual research, herbal and botanical distribution, traditional uses, phytochemistry, and pharmacology.

Ma J, Li Q, Wang T, Lu H, Liu J, Cai R Front Pharmacol. 2024; 14:1303902.

PMID: 38174223 PMC: 10762315. DOI: 10.3389/fphar.2023.1303902.

Biosynthesis of Functional Silver Nanoparticles Using Callus and Hairy Root Cultures of .

Yugay Y, Sorokina M, Grigorchuk V, Rusapetova T, Silantev V, Egorova A J Funct Biomater. 2023; 14(9).

PMID: 37754865 PMC: 10532211. DOI: 10.3390/jfb14090451.

Activities against Lung Cancer of Biosynthesized Silver Nanoparticles: A Review.

Mejia-Mendez J, Lopez-Mena E, Sanchez-Arreola E Biomedicines. 2023; 11(2).

PMID: 36830926 PMC: 9953519. DOI: 10.3390/biomedicines11020389.

References

Li S, Zhang H, Yao P, Sun H, Fong H . Rearranged taxanes from the bark of Taxus yunnanensis. J Nat Prod. 2000; 63(11):1488-91. DOI: 10.1021/np000118t. View

Singla A, Garg A, Aggarwal D . Paclitaxel and its formulations. Int J Pharm. 2002; 235(1-2):179-92. DOI: 10.1016/s0378-5173(01)00986-3. View

Shinozaki Y, Fukamiya N, Uchiyama C, Okano M, Tagahara K, Bastow K . Multidrug resistant cancer cells susceptibility to cytotoxic taxane diterpenes from Taxus yunnanensis and Taxus chinensis. Bioorg Med Chem Lett. 2002; 12(19):2785-8. DOI: 10.1016/s0960-894x(02)00528-0. View

Zhang C, Wu J . Effects of inoculum size and age on biomass growth and paclitaxel production of elicitor-treated Taxus yunnanensis cell cultures. Appl Microbiol Biotechnol. 2002; 60(4):396-402. DOI: 10.1007/s00253-002-1130-5. View

Nguyen N, Banskota A, Tezuka Y, Nobukawa T, Kadota S . Diterpenes and sesquiterpenes from the bark of Taxus yunnanensis. Phytochemistry. 2003; 64(6):1141-7. DOI: 10.1016/s0031-9422(03)00503-x. View

Wang B, Won S, Yu Z, Su C . Free radical scavenging and apoptotic effects of Cordyceps sinensis fractionated by supercritical carbon dioxide. Food Chem Toxicol. 2005; 43(4):543-52. DOI: 10.1016/j.fct.2004.12.008. View

Yin J, Tezuka Y, Subehan , Shi L, Nobukawa M, Nobukawa T . In vivo anti-osteoporotic activity of isotaxiresinol, a lignan from wood of Taxus yunnanensis. Phytomedicine. 2005; 13(1-2):37-42. DOI: 10.1016/j.phymed.2004.06.017. View

Banskota A, Nguyen N, Tezuka Y, Nobukawa T, Kadota S . Hypoglycemic effects of the wood of Taxus yunnanensis on streptozotocin-induced diabetic rats and its active components. Phytomedicine. 2005; 13(1-2):109-14. DOI: 10.1016/j.phymed.2004.01.015. View

Wang J, Zheng L, Wu J, Tan R . Involvement of nitric oxide in oxidative burst, phenylalanine ammonia-lyase activation and Taxol production induced by low-energy ultrasound in Taxus yunnanensis cell suspension cultures. Nitric Oxide. 2006; 15(4):351-8. DOI: 10.1016/j.niox.2006.04.261. View

10.

Gogoi S, Gopinath P, Paul A, Ramesh A, Ghosh S, Chattopadhyay A . Green fluorescent protein-expressing Escherichia coli as a model system for investigating the antimicrobial activities of silver nanoparticles. Langmuir. 2006; 22(22):9322-8. DOI: 10.1021/la060661v. View

11.

Koyama J, Morita I, Kobayashi N, Hirai K, Simamura E, Nobukawa T . Antiallergic activity of aqueous extracts and constituents of Taxus yunnanensis. Biol Pharm Bull. 2006; 29(11):2310-2. DOI: 10.1248/bpb.29.2310. View

12.

Kim J, Kuk E, Yu K, Kim J, Park S, Lee H . Antimicrobial effects of silver nanoparticles. Nanomedicine. 2007; 3(1):95-101. DOI: 10.1016/j.nano.2006.12.001. View

13.

Ruparelia J, Chatterjee A, Duttagupta S, Mukherji S . Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomater. 2008; 4(3):707-16. DOI: 10.1016/j.actbio.2007.11.006. View

14.

Sun C, Fang C, Stephen Z, Veiseh O, Hansen S, Lee D . Tumor-targeted drug delivery and MRI contrast enhancement by chlorotoxin-conjugated iron oxide nanoparticles. Nanomedicine (Lond). 2008; 3(4):495-505. PMC: 2890026. DOI: 10.2217/17435889.3.4.495. View

15.

Kim S, Choi J, Choi J, Chung K, Park K, Yi J . Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicol In Vitro. 2009; 23(6):1076-84. DOI: 10.1016/j.tiv.2009.06.001. View

16.

Thakkar K, Mhatre S, Parikh R . Biological synthesis of metallic nanoparticles. Nanomedicine. 2009; 6(2):257-62. DOI: 10.1016/j.nano.2009.07.002. View

17.

Kawata K, Osawa M, Okabe S . In vitro toxicity of silver nanoparticles at noncytotoxic doses to HepG2 human hepatoma cells. Environ Sci Technol. 2009; 43(15):6046-51. DOI: 10.1021/es900754q. View

18.

Liu Y, Li K, Pan J, Liu B, Feng S . Folic acid conjugated nanoparticles of mixed lipid monolayer shell and biodegradable polymer core for targeted delivery of Docetaxel. Biomaterials. 2009; 31(2):330-8. DOI: 10.1016/j.biomaterials.2009.09.036. View

19.

Sperling R, Parak W . Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles. Philos Trans A Math Phys Eng Sci. 2010; 368(1915):1333-83. DOI: 10.1098/rsta.2009.0273. View

20.

Martinez-Gutierrez F, Olive P, Banuelos A, Orrantia E, Nino N, Sanchez E . Synthesis, characterization, and evaluation of antimicrobial and cytotoxic effect of silver and titanium nanoparticles. Nanomedicine. 2010; 6(5):681-8. DOI: 10.1016/j.nano.2010.02.001. View