Optimization of Protease Production from Surface-modified Coffee Pulp Waste and Corncobs Using Bacillus Sp. by SSF

Overview

Journal 3 Biotech

Publisher Springer

Specialty Biotechnology

Date 2017 Mar 24

PMID 28330239

Citations 12

Authors

Selvam Kandasamy

Govarthanan Muthusamy

Senthilkumar Balakrishnan

Senbagam Duraisamy

Selvankumar Thangasamy

Kamala-Kannan Seralathan

Sudhakar Chinnappan

Affiliations

Soon will be listed here.

Abstract

The aim of the study was to identify new sources of substrate from agro-industrial waste for protease production using Bacillus sp., a local bacteria isolated from an agro-waste dumping site. The strain was identified as Bacillus sp. BT MASC 3 by 16S rRNA sequence followed by phylogenic analysis. Response surface methodology-based Box-Behnken design (BBD) was used to optimize the variables such as pH, incubation time, coffee pulp waste (CPW) and corncob (CC) substrate concentration. The BBD design showed a reasonable adjustment of the quadratic model with the experimental data. Statistics-based contour and 3-D plots were generated to evaluate the changes in the response surface and understand the relationship between the culture conditions and the enzyme yield. The maximum yield of protease production (920 U/mL) was achieved after 60 h of incubation with 3.0 g/L of CPW and 2.0 g/L of CC at pH 8 and temperature 37 °C in this study. The molecular mass of the purified enzyme was 46 kDa. The highest activity was obtained at 50 °C and pH 9 for the purified enzymes.

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