A Semisynthetic Atg3 Reveals That Acetylation Promotes Atg3 Membrane Binding and Atg8 Lipidation

Overview

Journal Nat Commun

Specialty Biology

Date 2017 Mar 23

PMID 28327644

Citations 24

Authors

Yi-Tong Li

Cong Yi

Chen-Chen Chen

Huan Lan

Man Pan

Shao-Jin Zhang

Yi-Chao Huang

Chao-Jian Guan

Yi-Ming Li

Li Yu

Lei Liu

Affiliations

Soon will be listed here.

Abstract

Acetylation of Atg3 regulates the lipidation of the protein Atg8 in autophagy. The molecular mechanism behind this important biochemical event remains to be elucidated. We describe the first semi-synthesis of homogeneous K19/K48-diacetylated Atg3 through sequential hydrazide-based native chemical ligation. In vitro reconstitution experiments with the semi-synthetic proteins confirm that Atg3 acetylation can promote the lipidation of Atg8. We find that acetylation of Atg3 enhances its binding to phosphatidylethanolamine-containing liposomes and to endoplasmic reticulum, through which it promotes the lipidation process.

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