Organisation of the Ribosomal RNA Genes in Streptomyces Coelicolor A3(2)

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1988 Feb 1

PMID 2832699

Citations 14

Authors

H A Baylis

M J Bibb

Affiliations

Soon will be listed here.

Abstract

Using Southern hybridisation of radiolabelled purified ribosomal RNAs to genomic DNA the ribosomal RNA genes of Streptomyces coelicolor A3(2) were shown to be present in six gene sets. Each gene set contains at least one copy of the 5 S, 16 S and 23 S sequences and in at least two cases these are arranged in the order 16 S - 23 S - 5 S. Three gene sets, rrnB, rrnD and rrnF, were isolated by screening a lambda library of S. coelicolor A3(2) DNA. The restriction map of one of these, rrnD, was determined and the nucleotide sequences corresponding to the three rRNAs were localised by Southern hybridisation. The gene order in rrnD is 16 S - 23 S - 5 S.

Citing Articles

Characterization of heterogeneous LSU rRNA profiles in Streptomyces coelicolor under different growth stages and conditions.

Kim H, Song W, Kim K, Lee K Curr Microbiol. 2008; 57(6):537-41.

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Characterization of the alternative sigma factor sigmaG in Streptomyces coelicolor A3(2).

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Variation in 16S-23S rRNA intergenic spacer regions in Photobacterium damselae: a mosaic-like structure.

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The ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) plays a conditional role in antibiotic production and morphological differentiation.

Chakraburtty R, BIBB M J Bacteriol. 1997; 179(18):5854-61.

PMID: 9294445 PMC: 179477. DOI: 10.1128/jb.179.18.5854-5861.1997.

Characterization of Streptomyces venezuelae ATCC 10595 rRNA gene clusters and cloning of rrnA.

La Farina M, Stira S, Mancuso R, Grisanti C J Bacteriol. 1996; 178(5):1480-3.

PMID: 8631730 PMC: 177827. DOI: 10.1128/jb.178.5.1480-1483.1996.

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