HSV-1 Interaction to 3-O-sulfated Heparan Sulfate in Mouse-derived DRG Explant and Profiles of Inflammatory Markers During Virus Infection

Overview

Journal J Neurovirol

Publisher Springer

Specialties Microbiology
Neurology

Date 2017 Mar 23

PMID 28326469

Citations 16

Authors

Harsh Sharthiya

Chanmoly Seng

T H van Kuppevelt

Vaibhav Tiwari

Michele Fornaro

Affiliations

Soon will be listed here.

Abstract

The molecular mechanism of herpes simplex virus (HSV) entry and the associated inflammatory response in the nervous system remain poorly understood. Using mouse-derived ex vivo dorsal root ganglia (DRG) explant model and single cell neurons (SCNs), in this study, we provided a visual evidence for the expression of heparan sulfate (HS) and 3-O-sulfated heparan sulfate (3-OS HS) followed by their interactions with HSV-1 glycoprotein B (gB) and glycoprotein D (gD) during cell entry. Upon heparanase treatment of DRG-derived SCN, a significant inhibition of HSV-1 entry was observed suggesting the involvement of HS role during viral entry. Finally, a cytokine array profile generated during HSV-1 infection in DRG explant indicated an enhanced expression of chemokines (LIX, TIMP-2, and M-CSF)-known regulators of HS. Taken together, these results highlight the significance of HS during HSV-1 entry in DRG explant. Further investigation is needed to understand which isoforms of 3-O-sulfotransferase (3-OST)-generated HS contributed during HSV-1 infection and associated cell damage.

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