Analysis and Isolation of Cytomegalovirus DNA by Field Inversion Gel Electrophoresis

Overview

Journal J Gen Virol

Specialty Microbiology

Date 1988 Mar 1

PMID 2832532

Citations 8

Authors

F M van den Berg

M Jiwa

R Rook

J L Geelen

Affiliations

Soon will be listed here.

Abstract

High molecular weight human cytomegalovirus (CMV) DNA was isolated from agarose-embedded infected human diploid cells by employing field inversion gel electrophoresis. A high yield of CMV DNA molecules was obtained within 1 week of infecting the cell culture. Labelling of the viral DNA with biotin by nick translation enabled the detection of CMV-infected cells in sections of paraffin-embedded human adrenal gland by in situ hybridization.

Citing Articles

Demonstration of a Sandhoff disease-associated autosomal 50-kb deletion by field inversion gel electrophoresis.

Bikker H, van den Berg F, Wolterman R, de Vijlder J, Bolhuis P Hum Genet. 1989; 81(3):287-8.

PMID: 2921040 DOI: 10.1007/BF00279006.

Detection of Campylobacter pylori in stomach tissue by DNA in situ hybridisation.

van den Berg F, Zijlmans H, Langenberg W, Rauws E, Schipper M J Clin Pathol. 1989; 42(9):995-1000.

PMID: 2794089 PMC: 501804. DOI: 10.1136/jcp.42.9.995.

Implausibility of an aetiological association between cytomegalovirus and Kaposi's sarcoma shown by four techniques.

van den Berg F, Schipper M, Jiwa M, Rook R, van De Rijke F, Tigges B J Clin Pathol. 1989; 42(2):128-31.

PMID: 2537856 PMC: 1141813. DOI: 10.1136/jcp.42.2.128.

Simultaneous application of in situ DNA hybridization and immunohistochemistry on one tissue section.

van der Loos C, Volkers H, Rook R, van den Berg F, Houthoff H Histochem J. 1989; 21(5):279-84.

PMID: 2476418 DOI: 10.1007/BF01757180.

On-line sorting of human chromosomes by centromeric index, and identification of sorted populations by GTG-banding and fluorescent in situ hybridization.

Boschman G, Rens W, Manders E, van Oven C, Barendsen G, Aten J Hum Genet. 1990; 85(1):41-8.

PMID: 2358302 DOI: 10.1007/BF00276324.