Bio-catalysis of Mango Industrial Waste by Newly Isolated Fusarium Sp. (PSTF1) for Pectinase Production

Overview

Journal 3 Biotech

Publisher Springer

Specialty Biotechnology

Date 2017 Mar 22

PMID 28324397

Citations 3

Authors

M Purnachandra Reddy

K V Saritha

Affiliations

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Abstract

The dried mango fruit processing industrial waste (MIW) used as carbon source for the production of pectinase from fungal strains. Eight fungal strains were isolated from MIW and screened for their ability to produce pectinase by pectin clear zone (PCZ) technique. Fusarium sp. (PSTF1) showed highest PCZ value of 52 mm. The physico-chemical characteristics of the medium were standardized for high production of pectinase. The highest production of pectinase in submerged fermentation observed at temperature-28 °C, pH-6.0, inoculum-size 0.6/25 ml, incubation-72 h, substrate concentration-0.6 g/100 ml, carbon source-fructose (1 %). The effect of different amino acids, vitamins also observed. Under these optimal conditions the highest activity 81.9718 µmol/ml of pectinase was observed. The Fusarium sp. (PSTF1) has been considered as the best pectinase producer in submerged fermentation of MIW. The cheap waste raw material used as best carbon source for high production of high value pectinase.

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