A Fifth Epstein-Barr Virus Nuclear Protein (EBNA3C) is Expressed in Latently Infected Growth-transformed Lymphocytes

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1988 Apr 1

PMID 2831394

Citations 42

Authors

L Petti

J Sample

F Wang

E Kieff

Affiliations

Soon will be listed here.

Abstract

Three distantly homologous neighboring long open reading frames in the Epstein-Barr virus (EBV) genome are preceded by short open reading frames. The leftmost short and long open reading frames encode EBNA3, a nuclear protein which is slightly smaller (145 kilodaltons [kDa]) than two other nuclear proteins (150 to 155 kDa) detected in Western blots (immunoblots) of latently infected cell protein (K. Hennessy, F. Wang, E. Woodland-Bushman, and E. Kieff, Proc. Natl. Acad. Sci. USA 83:5693-5697, 1986; I. Joab, D. T. Rowe, M. Bodescot, J.-C. Nicolas, P. J. Farrell, and M. Perricaudet, J. Virol. 61:3340-3344, 1987). We have demonstrated that the most rightward short (BERF3) and long (BERF4) open reading frames are spliced in frame at the 3' end of a 5-kilobase latently infected cell RNA and that this RNA begins within or upstream of the EBV long internal repeat. EBV-immune human antibodies specific for the long open reading frame translation product identified a 155-kDa protein on Western blots of latently infected cell protein and specifically reacted with large nonnucleolar nuclear granules in every latently infected cell. Expression of the cDNA in BALB/c 3T3 cells resulted in translation of full-size EBNA3C but had no effect on cell morphology, contact inhibition, or serum independence.

Citing Articles

Role of EBNA-3 Family Proteins in EBV Associated B-cell Lymphomagenesis.

Bhattacharjee S, Roy S, Bose P, Saha A Front Microbiol. 2016; 7:457.

PMID: 27092119 PMC: 4824013. DOI: 10.3389/fmicb.2016.00457.

Epstein-Barr virus nuclear antigen 3A promotes cellular proliferation by repression of the cyclin-dependent kinase inhibitor p21WAF1/CIP1.

Tursiella M, Bowman E, Wanzeck K, Throm R, Liao J, Zhu J PLoS Pathog. 2014; 10(10):e1004415.

PMID: 25275486 PMC: 4183747. DOI: 10.1371/journal.ppat.1004415.

Impact of EBV essential nuclear protein EBNA-3C on B-cell proliferation and apoptosis.

Saha A, Robertson E Future Microbiol. 2013; 8(3):323-52.

PMID: 23464371 PMC: 5965686. DOI: 10.2217/fmb.12.147.

Occupancy of chromatin organizers in the Epstein-Barr virus genome.

Holdorf M, Cooper S, Yamamoto K, Miranda J Virology. 2011; 415(1):1-5.

PMID: 21550623 PMC: 3808970. DOI: 10.1016/j.virol.2011.04.004.

Multiple roles of Epstein-Barr virus SM protein in lytic replication.

Han Z, Marendy E, Wang Y, Yuan J, Sample J, Swaminathan S J Virol. 2007; 81(8):4058-69.

PMID: 17287267 PMC: 1866120. DOI: 10.1128/JVI.02665-06.

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