Spatial and Temporal Coincidence of Induction Processes and Gap-junctional Communication in Patella Vulgata (Mollusca, Gastropoda)

Overview

Journal Rouxs Arch Dev Biol

Specialty Biology

Date 2017 Mar 18

PMID 28306091

Citations 2

Authors

P Damen

W J A G Dictus

Affiliations

Soon will be listed here.

Abstract

In the early development of the gastropod mollusc Patella vulgata, two inductive processes take place between the animal and vegetal hemispheres. One is the induction of the vegetally-located stem cell of the mesoderm (the 3D macromere) by the first-quartet micromeres (which are located in the animal hemisphere). The other is the induction of dorsoventral symmetry in the first-quartet micromeres by the 3D macromere. As a consequence of the latter induction process, a dorsoventrally-organised prototroch is formed. In this paper, the moment of 3D induction is determined by deleting the inducing first-quartet micromeres at successive stages of development. Furthermore, the role of gap junctions in the two above-mentioned induction processes is investigated. This was done by studying lucifer yellow dye transfer between the 3D macromere and the first-quartet micromeres, in relation to these two induction processes. Analysis of the pattern of dye-transfer in vivo, in sections and with confocal laser scanning microscopy demonstrated that the moment of dye transfer coincides with the moment of 3D induction and with the moment of induction of the dorsoventral pattern of the prototroch. This indicates that gap-junctional communication may be involved in these two induction processes.

Citing Articles

Functional evidence that Activin/Nodal signaling is required for establishing the dorsal-ventral axis in the annelid .

Lanza A, Seaver E Development. 2020; 147(18).

PMID: 32967906 PMC: 7522025. DOI: 10.1242/dev.189373.

Activin/Nodal signaling mediates dorsal-ventral axis formation before third quartet formation in embryos of the annelid .

Lanza A, Seaver E Evodevo. 2020; 11:17.

PMID: 32788949 PMC: 7418201. DOI: 10.1186/s13227-020-00161-y.

References

van den Biggelaar J, Guerrier P . Dorsoventral polarity and mesentoblast determination as concomitant results of cellular interactions in the mollusk Patella vulgata. Dev Biol. 1979; 68(2):462-71. DOI: 10.1016/0012-1606(79)90218-5. View

Spurr A . A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969; 26(1):31-43. DOI: 10.1016/s0022-5320(69)90033-1. View

VAN DER WAL U, Dohmen M . A method for the orientation of small and delicate objects in embedding media for light and electron microscopy. Stain Technol. 1978; 53(1):56-8. View

Dorresteijn A, Wagemaker H, de Laat S, van den Biggelaar J . Dye-coupling between blastomeres in early embryos ofPatella vulgata (mollusca, gastropoda): Its relevance for cell determination. Wilehm Roux Arch Dev Biol. 2017; 192(5):262-269. DOI: 10.1007/BF00848658. View

de Laat S, Tertoolen L, Dorresteijn A, van den Biggelaar J . Intercellular communication patterns are involved in cell determination in early molluscan development. Nature. 1980; 287(5782):546-8. DOI: 10.1038/287546a0. View

Damen P, Dictus W . Organiser role of the stem cell of the mesoderm in prototroch patterning in Patella vulgata (Mollusca, Gastropoda). Mech Dev. 1996; 56(1-2):41-60. DOI: 10.1016/0925-4773(96)00510-2. View

Dorresteijn A, Bilinski S, van den Biggelaar J, Bluemink J . The presence of gap junctions during early Patella embryogenesis: an electron microscopical study. Dev Biol. 1982; 91(2):397-401. DOI: 10.1016/0012-1606(82)90045-8. View

Simpson I, Rose B, Loewenstein W . Size limit of molecules permeating the junctional membrane channels. Science. 1977; 195(4275):294-6. DOI: 10.1126/science.831276. View

Damen P, Dictus W . Cell lineage of the prototroch of Patella vulgata (Gastropoda, Mollusca). Dev Biol. 1994; 162(2):364-83. DOI: 10.1006/dbio.1994.1094. View

10.

Muller L, Jacks T . Rapid chemical dehydration of samples for electron microscopic examinations. J Histochem Cytochem. 1975; 23(2):107-10. DOI: 10.1177/23.2.1117127. View

11.

van den Biggelaar J . Development of dorsoventral polarity and mesentoblast determination in Patella vulgata. J Morphol. 1977; 154(1):157-86. DOI: 10.1002/jmor.1051540111. View

12.

Damen P, Dictus W . Cell-lineage analysis of the prototroch of the gastropod molluscPatella vulgata shows conditional specification of some trochoblasts. Rouxs Arch Dev Biol. 2017; 203(4):187-198. DOI: 10.1007/BF00636334. View

13.

Fraser S, Green C, Bode H, Gilula N . Selective disruption of gap junctional communication interferes with a patterning process in hydra. Science. 1987; 237(4810):49-55. DOI: 10.1126/science.3037697. View

14.

Boring L . Cell-cell interactions determine the dorsoventral axis in embryos of an equally cleaving opisthobranch mollusc. Dev Biol. 1989; 136(1):239-53. DOI: 10.1016/0012-1606(89)90145-0. View

15.

Arnolds W, van den Biggelaar J, Verdonk N . Spatial aspects of cell interactions involved in the determination of dorsoventral polarity in equally cleaving gastropods and regulative abilities of their embryos, as studied by micromere deletions inLymnaea andPatella. Wilehm Roux Arch Dev Biol. 2017; 192(2):75-85. DOI: 10.1007/BF00848483. View

16.

Serras F, Damen P, Dictus W, Notenboom R, van den Biggelaar J . Communication compartments in the ectoderm of embryos of Patella vulgata. Rouxs Arch Dev Biol. 2017; 198(4):191-200. DOI: 10.1007/BF00375905. View

17.

Kuhtreiber W, van Til E, van Dongen C . Monensin interferes with the determination of the mesodermal cell line in embryos of Patella vulgata. Rouxs Arch Dev Biol. 2017; 197(1):10-18. DOI: 10.1007/BF00376036. View