Fission Yeast Tmsl Protein Abrogates Normal Development in Xenopus Laevis Embryos

Overview

Journal Rouxs Arch Dev Biol

Specialty Biology

Date 2017 Mar 18

PMID 28305960

Authors

Peter Wagner

Michael Hoever

Katrin Appel

Walter Knochel

Mathias Montenarh

Affiliations

Soon will be listed here.

Abstract

Recently we cloned tms1 (a putative dehydrogenase) by complementation of a human tumour-derived mutant p53 induced growth arrest in fission yeast. Microinjection of purified tmsl protein into Xenopus laevis embryos abrogated normal embryo development by causing cleavage retardation or cleavage arrest of injected blastomeres in a concentration dependant manner, whereas injection of specific affinity purified tms1 antiserum showed no significant morphological defects. Microinjection of tms1 protein together with affinity purified tms1 antibody resulted in a significantly reduced number of cleavage arrested embryos.

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