Distribution and Redistribution of Pigment Granules in the Development of Sea Urchin Embryos

Overview

Journal Wilehm Roux Arch Dev Biol

Specialty Anatomy & Histology

Date 2017 Mar 18

PMID 28305346

Citations 2

Authors

Yuichiro Tanaka

Affiliations

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Abstract

Pigment granules (PGs) are embeded in the cortex of embryos of the Japanese sea urchins,Hemicentrotus pulcherrimus and Anthocidaris crassispina. PGs in the cortex actively retreated from the vegetal pole area at the 4-cell stage and then a notable PG-distribution gradient formed along the egg axis (the polar redistribution of PGs). The polar redistribution of PGs in the cortex occurred at the same time after fertilization even in solutions of microtubule disrupting reagents such as Colcemid, vinblastine sulfate or griseofulvin. Consequently, the polar redistribution of PGs was not associated with the microtubules. However, the polar redistribution of PGs was interrupted in seawater containing cytochalasin B (CB), dithiothreitol (DTT) or tetracaine, and the distribution pattern of PGs in the cortex was definitely disturbed. Moreover, CB, DTT and tetracaine altered the division pattern of vegetal blastomeres at the 4th cleavage which is normally unequal so that all the blastomeres divided equally. Microtubule disrupting reagents did not have such an effect on the cleavage pattern. Thus the cortical movement along the egg axis reflected by the polar redistribution of PGs seems to correlate with the micromere formation.

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References

Belanger A, RUSTAD R . Movements of echinochrome granules during the early development of sea urchin eggs. Nat New Biol. 1972; 239(90):81-3. DOI: 10.1038/newbio239081a0. View

Nicolson G . Cell shape changes and transmembrane receptor uncoupling induced by tertiary amine local anesthetic. J Supramol Struct. 1976; 5(1):65-72. DOI: 10.1002/jss.400050107. View

Ikeda M . Behaviour of sulfhydryl groups of sea urchin eggs under the blockage of cell division by UV and heat shock. Exp Cell Res. 1965; 40(2):282-91. DOI: 10.1016/0014-4827(65)90261-2. View

REBHUN L . Polarized intracellular particle transport: saltatory movements and cytoplasmic streaming. Int Rev Cytol. 1972; 32:93-137. DOI: 10.1016/s0074-7696(08)60339-3. View

Dan K, Ikeda M . On the system controlling the time fo micromere formation in sea urchin embryos. Dev Growth Differ. 1971; 13(4):285-301. DOI: 10.1111/j.1440-169x.1971.00285.x. View

Papahadjopoulos D . Studies on the mechanism of action of local anesthetics with phospholipid model membranes. Biochim Biophys Acta. 1972; 265(2):169-86. DOI: 10.1016/0304-4157(72)90001-9. View

Tyler A . Crystalline Echinochrome and Spinochrome: Their Failure to Stimulate the Respiration of Eggs and of Sperm of Strongylocentrotus. Proc Natl Acad Sci U S A. 1939; 25(10):523-8. PMC: 1077960. DOI: 10.1073/pnas.25.10.523. View

Spudich A, Spudich J . Actin in triton-treated cortical preparations of unfertilized and fertilized sea urchin eggs. J Cell Biol. 1979; 82(1):212-26. PMC: 2110418. DOI: 10.1083/jcb.82.1.212. View

Brachet J, Hubert E . Studies on nucleocytoplasmic interactions during early amphibian development. I. Localized destruction of the egg cortex. J Embryol Exp Morphol. 1972; 27(1):121-45. View

10.

Wolfe J, Rodriguez L . Inhibition of nuclear reconstruction by dithiothreitol. J Cell Biol. 1974; 60(2):497-502. PMC: 2109167. DOI: 10.1083/jcb.60.2.497. View

11.

Kane R . Preparation and purification of polymerized actin from sea urchin egg extracts. J Cell Biol. 1975; 66(2):305-15. PMC: 2109559. DOI: 10.1083/jcb.66.2.305. View

12.

REBHUN L . Structural aspects of saltatory particle movement. J Gen Physiol. 1967; 50(6):Suppl:223-39. PMC: 2225743. DOI: 10.1085/jgp.50.6.223. View

13.

Schroeder T . Actin in dividing cells: contractile ring filaments bind heavy meromyosin. Proc Natl Acad Sci U S A. 1973; 70(6):1688-92. PMC: 433573. DOI: 10.1073/pnas.70.6.1688. View

14.

Czihak G . The role of astral rays in early cleavage of sea urchin eggs. Exp Cell Res. 1974; 83(2):424-6. DOI: 10.1016/0014-4827(74)90361-9. View

15.

Schroeder T . The contractile ring. II. Determining its brief existence, volumetric changes, and vital role in cleaving Arbacia eggs. J Cell Biol. 1972; 53(2):419-34. PMC: 2108733. DOI: 10.1083/jcb.53.2.419. View

16.

Curtis A . Morphogenetic interactions before gastrulation in the amphibian, Xenopus laevis--the cortical field. J Embryol Exp Morphol. 1962; 10:410-22. View

17.

Schroeder T . Expressions of the prefertilization polar axis in sea urchin eggs. Dev Biol. 1980; 79(2):428-43. DOI: 10.1016/0012-1606(80)90128-1. View

18.

Pasteels J . THE MORPHOGENETIC ROLE OF THE CORTEX OF THE AMPHIBIAN EGG. Adv Morphog. 1964; 4:363-88. DOI: 10.1016/b978-1-4831-9950-4.50012-6. View