A Glycoprotein in the Accessory Cell of the Echinoid Ovary and Its Role in Vitellogenesis

Overview

Journal Rouxs Arch Dev Biol

Specialty Biology

Date 2017 Mar 18

PMID 28305279

Citations 2

Authors

Hironobu Ozaki

Osamu Moriya

Frederick Elton Harrington

Affiliations

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Abstract

A high-molecular-weight glycoprotein with a sedimentation coefficient of 22.6 has been isolated and characterized from the accessory cells in the previtellogenic ovary of the echinoid Dendraster excentricus. This glycoprotein is similar to the major yolk glycoprotein of the mature egg in its electrophoretic mobility under non-denaturing conditions, high mannose-type glycan, amino acid composition, constitutive glycopeptides, and immunological determinants. Previous histological and electron microscopical analyses led to the hypothesis that vitellogenesis involves a translocation of material from the accessory cell in the ovary to the oocyte. Because of the close similarities of the accessory cell glycoprotein to the yolk glycoprotein of the mature egg, we conclude that the glycoprotein in the accessory cell is a precursor to the major glycoprotein of the egg yolk. This conclusion is further supported by our additional finding that the accessory cell of another echinoid, Strongylocentrotus purpuratus, also contains a high-molecular-weight (24 S) glycoprotein which shows similarities to the yolk glycoprotein of the mature egg in the carbohydrate moiety and the constitutive glycopeptides.

Citing Articles

Glycobiology of reproductive processes in marine animals: the state of the art.

Gallo A, Costantini M Mar Drugs. 2012; 10(12):2861-92.

PMID: 23247316 PMC: 3528131. DOI: 10.3390/md10122861.

Endocrine disrupting compounds and echinoderms: new ecotoxicological sentinels for the marine ecosystem.

Sugni M, Mozzi D, Barbaglio A, Bonasoro F, Candia Carnevali M Ecotoxicology. 2007; 16(1):95-108.

PMID: 17253161 DOI: 10.1007/s10646-006-0119-8.

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